Product Overview

WS RTL Reverse Transcriptase 2.0 (Glycerol-Free) is a warm-start reverse transcriptase engineered with reversible aptamer blocking technology. The enzyme remains effectively inhibited at lower temperatures, minimizing non-specific amplification during reaction setup, and is rapidly activated at working temperatures to deliver efficient reverse transcription performance.

The glycerol-free formulation offers greater flexibility for assay development and lyophilized reagent preparation. Combined with excellent amplification speed, sensitivity, and reaction consistency, WS RTL Reverse Transcriptase 2.0 (Glycerol-Free) is well suited for molecular diagnostic assays, including RT-LAMP and other isothermal amplification workflows.

Key Features

Reversible Aptamer-Based Warm Start Technology

Aptamer blocking effectively suppresses enzyme activity at lower temperatures. Upon reaching the reaction temperature, the aptamer dissociates from the enzyme, enabling rapid activation and reducing non-specific amplification.

Enhanced Specificity

WS RTL Reverse Transcriptase 2.0 exhibits significantly lower enzymatic activity at 35°C after aptamer blocking, helping improve reaction specificity and assay reliability.

High Sensitivity Detection

Maintains stable amplification performance across different target concentrations, supporting sensitive RNA detection in low-copy-number samples.

Fast Amplification Performance

Delivers faster amplification compared with competing reverse transcriptase under the same testing conditions, helping shorten overall assay turnaround time.

Consistent Amplification Curves

Produces highly reproducible amplification profiles across a wide concentration range, ensuring reliable assay results and simplified data interpretation.

Glycerol-Free Formulation

Suitable for lyophilization processes and flexible assay development, supporting a variety of molecular diagnostic applications.

Applications

• RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification)

• Warm-start RNA amplification assays

• Molecular diagnostic assay development

• Point-of-care testing (POCT)

• Infectious disease detection

• RNA target detection and quantification

• Lyophilized molecular diagnostic reagent development

Performance Data

Reversible Aptamer Blocking Improves Specificity

Reversible Aptamer Blocking

Similar to antibody-based methodologies, aptamer modification is a method of enzymatic activity blocking. The enzyme is inactivated through tight binding between the aptamer and the polymerase at lower temperatures. At working temperatures, the aptamer dissociates from the enzyme, activating its catalytic function.

After aptamer-based blocking, WS RTL Reverse Transcriptase 2. 0 shows significantly reduced enzymatic activity at 35 °C, indicating improved blocking efficiency. This is beneficial for controlling non-specific amplification.

High Sensitivity and Rapid Amplification

Across varying target concentrations, HH WS-RTL Reverse Transcriptase 2. 0 maintains consistent curve quality. Under identical conditions, competitor products show slower amplification speeds, lower sensitivity, and inconsistent curve profiles.

FAQ

Q1: What is WS RTL 2.0?

A: It is a glycerol-free version of RTL Reverse Transcriptase 2.0 designed for lyophilized and POCT applications.

Q2: Is it suitable for freeze-dried diagnostic kits?

A: Yes. It is optimized for dry reagent systems and maintains stable performance after lyophilization.

Q3: Does it retain RNase H activity?

A: Yes. It retains the full enzymatic profile of RTL 2.0.

Q4: What applications is it best suited for?

A: RT-LAMP, RT-PCR, and integrated POCT diagnostic platforms.