Product Description
Peptide - N-Glycosidase F (PNGase F) is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.
Product Information
Product Name | Cat. No | Size |
Rapid N-Glycan Kit | PNGase F | 50μL |
Applications
This enzyme is useful for removal of carbohydrate residues from proteins.
Unit definition
One unit(U) is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37 °C in a total reaction volume of 10 µL.