Customized mRNA CRO Services

Hzymes offers comprehensive solutions for mRNA vaccine raw material enzymes, process development, CRO synthesis, and third-party quality inspection, based on proprietary raw material enzymes, a unique high-throughput enzymatic reaction condition screening system, and an mRNA synthesis process development platform.

Service Content

Service Advantages

One-stop Service: Provides a comprehensive solution for concept validation in mRNA vaccine and drug development, accelerating the process from early-stage R&D to commercial production (more information available on the official website: www.hzymesbiotech.com).
Rich Project Experience: Offers guidance on antigen design and mRNA sequence design for clients.
Comprehensive Quality Control Platform: Equipped with quality control platforms such as HPLC, CE, and LC-MS, along with supporting methods, and a well-established quality management system.
Fast Delivery Cycle and Controllable Process Development Costs: Leverages proprietary raw material enzymes and extensive project experience, along with a high-throughput screening system, to provide advantages in project cycle and cost for CMC process development and optimization services.
Flexible and Open Technical Cooperation: Deep collaboration with CDMO partners to meet the diverse needs of clients at different stages.

Case sharing

Multiple mRNA vaccine concept verification example
• Step 1: Sequence Design  
Use multiple sequence design software to perform codon optimization on the amino acid sequences, self-cleaving sequences, and signal peptide sequences of proteins X and Y.
• Step 2: Gene Synthesis
Optimize and enhance the supercoiled ratio of the plasmid.
• Step 3: In Vitro Transcription
Obtain high-purity mRNA stock solution.
• Step 4: Translation Level Detection
• Step 5: mRNA-LNP Formulation

Example of mRNA Synthesis Process Development and High-Throughput Screening

• The production process of mRNA stock solution is complex, involving over 8 major categories of key raw materials in a one-step method, with multiple strategies for purification.
• High-throughput methods are utilized for the development of DNA template production processes.
• The high-throughput formulation screening platform is used for high-throughput IVT (in vitro transcription) and purification process development, reducing development time and improving research efficiency.

In Vivo and In Vitro Detection Method Development
DNA Template Screening
IVT System Ratio Screening
Purification Strategy Screening
Hundred-Milligram and Gram-Scale Scale-Up Testing

Example of Gram-Scale mRNA Stock Solution Scale-Up Production

mRNA sample testing service example