Product Overview

Hzymes Biotech has upgraded its host residual DNA detection products, covering multiple cell types such as  CHO, E. coli, Vero, HEK293, Pichia pastoris, and mycoplasma. The primer and probe sequences of the kit are from the pharmacopoeia, so you don’t need to do a full set of validation experiments, just need to confirm the method. Developed based on a quantitative PCR method,   LODs can reach 0. 003 pg/μL with no cross-reactivity with other DNA, reducing false positives in the assay reaction. Strict production management system,   batch-to-batch difference controllable CV< 10%. Provide a complete set of solutions for manual extraction and automatic instrument extraction< /b40> , which can quickly complete the detection within 2 hours and ensure the accuracy of quantitative detection.

Key Features

Performance Data

Standard Amplification Curve and Linearity Plot.

Actual Sample Detection Results

Product Stability Parameters

Product usage

Using the qPCR detection principle of TaqMan fluorescent probe, it is used to quantify the residual plasmid DNA in cell lines used in biological production processes.

 
The Plasmid Residual DNA Detection Kit quantitatively determines the residual plasmid DNA in samples through the shared KanR (Prokaryotic resistance) gene sequence of plasmids. The detection is fast, specific, and reliable in performance.

 
This testing method can be traced back to the General Principles of Methods in the United States Pharmacopoeia< 1130> and the General Principles of Methods in the Chinese Pharmacopoeia< 3407>

Transportation and storage methods

1. Expiry date: 24 months under specified storage conditions.

 
2. Transport and storage: for long time storage, please place at -25~-15 ℃ , during transport, please place in dry ice or ice packs.