Product Overview

RTL Reverse Transcriptase 2.0 (Glycerol-Free) is a thermostable reverse transcriptase designed for RNA-to-cDNA synthesis under isothermal amplification conditions. The enzyme possesses RNA-dependent DNA polymerase activity and RNase H activity, enabling efficient reverse transcription of RNA templates into complementary DNA.

Compared with conventional reverse transcriptases, RTL Reverse Transcriptase 2.0 demonstrates enhanced activity at elevated temperatures and maintains robust performance under RT-LAMP reaction conditions (60–65°C). The glycerol-free formulation is specifically developed for lyophilization workflows and freeze-dried RT-LAMP reagent development, while maintaining excellent thermal stability, freeze-thaw stability, and inhibitor tolerance.

RTL Reverse Transcriptase 2.0 (Glycerol-Free) is suitable for first-strand cDNA synthesis, RT-LAMP assay development, molecular diagnostics, and point-of-care testing (POCT) applications.

Key Features

Optimized for RT-LAMP Reactions

Maintains high reverse transcription activity at 60–65°C, matching typical RT-LAMP reaction temperatures and supporting rapid RNA amplification workflows.

High Reverse Transcription Efficiency

Efficient cDNA synthesis can be completed within minutes, enabling sensitive RNA detection in RT-LAMP assays.

Excellent Thermal Stability

Retains strong enzymatic activity after incubation at elevated temperatures, supporting reliable performance during assay development and storage.

Glycerol-Free for Lyophilization

Designed for freeze-dried reagent formulation and lyophilized RT-LAMP kit development without the need for glycerol-containing enzyme preparations.

Outstanding Freeze-Thaw Stability

Maintains consistent activity after multiple freeze-thaw cycles, supporting manufacturing, transportation, and routine laboratory use.

Strong Inhibitor Tolerance

Demonstrates tolerance to common sample and extraction-derived inhibitors, including guanidine salts, sodium chloride, and whole blood components, making it suitable for direct amplification applications.

Applications

• RT-LAMP assay development

• Lyophilized RT-LAMP reagent formulation

• Isothermal nucleic acid amplification

• First-strand cDNA synthesis

• RNA pathogen detection

• Molecular diagnostic assay development

• Point-of-care testing (POCT)

• Direct amplification workflows

• Research and clinical assay development

Performance Data

High Reverse Transcription Activity at RT-LAMP Temperatures

The activity of RTL reverse transcriptase 2. 0 at different temperatures.

Hzymes Biotech's RTL reverse transcriptase 2. 0 (HMD5302) has excellent heat resistance, and its optimum activity range perfectly adapts to the reaction conditions of RT-LAMP (60-65 °C). Its reverse transcription efficiency is high, and can be completed within 1-3 minutes.

Enhanced Thermal Stability

Compared with Company N, Haymes Biotech's RTL reverse transcriptase 2. 0 (HMD5302) has higher activity and better stability when incubated at 37 °C and 50 °C for 30 min and 10 min.

Suitable for Lyophilized Reagent Development

Haymes Biotech's RTL reverse transcriptase 2. 0 (HMD5302), incubated at 25°C for 30 days and incubated at 37°C for 7 days, the reverse transcription performance of RT-LAMP did not change, and the activity maintained the same. To ensure the development of RT-LAMP lyophilized reagents, the activity of glycerol-free RTL reverse transcriptase 2. 0 (HMD5302) remained unchanged after repeated freeze-thaw cycles 50 times.

Strong Tolerance to Common Inhibitors

Guanidine hydrochloride and guanidine isothiocyanate are common nucleic acid extraction residues. RTL reverse transcriptase 2. 0 (HMD5202) activity is higher than that of Company N under the same inhibition concentration. It is better than competing products with NaCl and whole blood (including single components). It is more suitable for various application scenarios, such as direct amplification, and easier for the subsequent development of RT-LAMP reagent.

FAQ

Q1: What is RTL Reverse Transcriptase 2.0 used for?

A: It is used for cDNA synthesis from RNA templates in RT-PCR, RT-qPCR, and molecular diagnostics.

Q2: Does RTL 2.0 have RNase H activity?

A: Yes. It has RNase H activity, enabling efficient RNA template processing during reverse transcription.

Q3: How does it compare with MMLV reverse transcriptase?

A: RTL 2.0 contains RNase H activity, whereas many MMLV variants do not. Substitution depends on system optimization and should be experimentally validated.

Q4: What are the advantages of RTL 2.0?

A: It provides improved reaction robustness, better RNA template processing, and strong performance in RT-PCR systems.