Specification
| Appearance | White amorphous powder, lyophilized |
| Purity | ≥90% (from SDS-PAGE) |
| Activity | ≥5.8 U/mg solid |
| Catalase | ≤0.03% |
| Creatinine amidohydrolase | ≤0.01% |
| Sarcosine oxidase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Properties
| EC number | 3.5.3.3 (Recombinantfrom microorganism) | |
| Molecular weight | 48 kDa (SDS-PAGE) | |
| Isoelectric point | 4.6 | |
| Michaelis constants | 8.81×10-3M (Creatine) | |
| Inhibitors | Ag+,Cu2+,Hg2+ | |
| Optimum pH | 8 | Fig.1 |
| Optimum temperature | 45℃ | Fig.2 |
| pH stability | pH 4.5-10.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 50℃ (pH 7.5,30 min) | Fig.4 |
| Stabilizers | EDTA,sugar | |
Applications
This enzyme is useful for the enzymatic determination of creatinine when coupled with creatinine amidohydrolase and sarcosine oxidase.
Unit Definition
One unit (U) is the amount of enzyme that produces 1 μmol of yellow dye per min under the conditions described below.
