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Home · Products · Products · Biochemical Diagnostics · Biochemical Diagnostic Enzymes · Lactate Metabolism · Lactate Oxidase ·
Lactate Oxidase
Lactate Oxidase

CAS：9028-72-2

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3 Ku
HH6201-01
100 Ku
HH6201-02
500 Ku
HH6201-03
1000 Ku
HH6201-04

Cat. No: HH6201 

Activity: ≥45. 0 U/mg 

Stability: Store at -25 ～ -15 ℃ for 12 months could maintain more than 90% activity.

Technical Support

Preparation and specification


Appearance

Yellow amorphous powder, lyophilized

Purity(SDS-PAGE)

≥90.0%

Enzyme powder specific activity

≥45.0 U/mg

Pyruvate oxidase

≤0.001%

Choline oxidase

≤0.001%

Uricase

≤0.001%

Glucose oxidase

≤0.001%

Properties

Source

Microorganisms

Classification

EC 1. 1.3.2 (Recombinant from microorganism)

Molecular weight

42kDa (SDS-PAGE)

Isoelectric point

pH 4.6

KmValue

7.5 ×10-4 M (L-Lactate)

Optimum pH

6.0-7.0

Fig. 1

Optimum temperature

50 °C

Fig. 2

pH stability

pH 6.0-8.5 (25 °C, 16 h)

Fig. 3

Thermal stability

Below 50 °C（pH 7.0, 30min）

Fig. 4

Stability

Store at -25～ -15 ℃ for 12 months could maintain more than 90% activity

Fig. 5



Applications


This enzyme is useful for enzymatic determination of lactic acid.

Fig. 1 Optimum pH


Buffer solution: plH 4.5-5.5, Acetate: pHH6.0-8.0, Na-phosphate; pH 8.0-9.0, Tris-HCl:pH 9.0-10.0, Glycine-NaOH.
Enzyme concentration: 1 mg/mL



Fig. 2 Optimum temperature


Rcaction in 20 mM K-phosphate buffer pH 7.0.

Enzyme concentration: 1 mg/mL


Fig. 3 pH stability


25°C, 16 h-trcatment with 50 mM buffer 

solution: pH 4.5-5.5, Acctate: pH 6.0-8.0,Na-phosphate; pH 8.0-9.0, Tris-HC1; pH9.0-10.0, Glycine-NaOH.

Enzyme concentration: 1 mg/mL



Fig. 4 Thermal stability


30 min-trcatment with 50mM K-phosphatebuffer plH 7.0.
Enzyme concentration: 1 mg/mL


Fig. 5 Stability (store at -25～-15℃)



Assay Principle


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D-Lactatedehy Drogenase

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