Specification
| Appearance | Yellow amorphous powder, lyophilized |
| Purity(SDS-PAGE) | ≥90% |
| Enzyme powder specific activity | ≥45.0 U/mg |
| Pyruvate oxidase | ≤0.001% |
Choline oxidase |
≤0.001% |
| Uric acid |
≤0.001% |
| Glucose Oxidase | ≤0.001% |
Properties
| Source | Microorganisms | |
| Classification | EC1.1.3.2 (Recombinant from microorganism) | |
| Molecular weight | 42kDa (SDS-PAGE) | |
| Isoelectric point | pH4.6 | |
| KmValue | 7.5×10-4M (L-Lactate) | |
| Optimum pH | 6.0-7.0 | Fig.1 |
| Optimum temperature | 50°C | Fig.2 |
| pH stability | pH 6.0-8.5 (25°C,16h) | Fig.3 |
| Thermal stability | Below 50°C（pH7.0,30min） | Fig.4 |
| Stability | Store at -25 ～ -15 ℃ for 12 months could maintain more than 90% activity | Fig.5 |
Applications
This enzyme is useful for enzymatic determination of lactic acid.
Unit definition
One unit is defined as the amount of enzyme which produces 1 μmol of H2O2 per minute under the conditions described below
