Product description
Peptide - N-Glycosidase F (PNGase F) is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.
Specification
| Appearance | Colorless Liquid |
| Protein purity | ≥95% (from SDS-PAGE) |
| Activity | ≥500,000 U/mL |
| Exoglycosidase | No activity could be detected (N D) |
| Endoglycosidase F1 | ND |
| Endoglycosidase F2 | ND |
| Endoglycosidase F3 | ND |
| Endoglycosidase H | ND |
| Protease | ND |
Properties
| EC number | 3.5.1.52 (Recombinant from microorganism) | |
| Molecular weight | 35 kDa (SDS-PAGE) | |
| Isoelectric point | 8.14 | |
| Optimum pH | 7.0-8.0 | |
| Optimum temperature | 65 °C | |
| Substrate specificity | Cleaving glycosidic bonds between GlcNAc and asparagine residues | Fig. 1 |
| recognition sites | N-linked glycans unless containing α1-3 fucose | Fig. 2 |
| Activators | DTT | |
| Inhibitor | SDS | |
| Storage temperature | -25～-15 ℃ | |
| Heat Inactivation | A 20 µL reaction mixture containing 1 µL of PNGase F is inactivated by incubation at 75 °C for 10 minutes. |
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Applications
This enzyme is useful for removal of carbohydrate residues from proteins.
Unit definition
One unit(U) is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37 °C in a total reaction volume of 10 µL.
