Hot Start Plus Taq DNA Polymerase (Antibody modified)

Product Overview

Hot Start Plus Taq DNA Polymerase (Antibody Modified) is a hot-start Taq DNA polymerase designed for rapid and high-specificity DNA amplification. The enzyme activity is tightly inhibited at room temperature through a specific antibody-based blocking mechanism, which suppresses both 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity prior to thermal activation.

Upon heating at 95°C for approximately 30 seconds, the antibody is released and full enzyme activity is restored.

This dual-blocking system helps reduce non-specific amplification and improves reagent stability in pre-mixed systems containing primers and probes. It also helps maintain amplification efficiency by minimizing degradation-related signal loss in key reaction components.

This product is suitable for fast PCR and multiplex quantitative PCR (qPCR) applications using DNA templates, and supports pre-mixed assay formats for improved workflow convenience and reduced handling time.

Key Features

High dual-blocking efficiency

A specific antibody system effectively blocks both 5’→3’ polymerase activity and 5’→3’ exonuclease activity under non-activating conditions, helping improve reaction specificity.

Rapid activation

Enzyme activity is quickly restored by heating at 95°C for approximately 30 seconds, supporting fast PCR workflows.

Balanced multiplex amplification

Provides stable amplification performance and consistent amplification curves in multiplex PCR and probe-based qPCR assays.

Improved premix stability

Suitable for primer-probe premixed systems. Stability testing at 37°C for 7 days shows no significant performance difference compared with samples stored at -20°C under recommended conditions.

Applications

• Routine PCR assays

• Hot-start PCR

• TaqMan probe-based qPCR

• Multiplex PCR

• Amplification of genomic DNA and plasmid DNA templates

• Broad-range PCR of bacterial 16S rRNA genes

• Pre-mixed PCR and qPCR systems

Performance Data

High dual-blocking efficiency

HMD0214 uses a specific antibody-based system to tightly inhibit both 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity under non-activating conditions, helping maintain high reaction specificity.

Rapid activation

More than 90% of enzyme activity can be restored after activation at 95°C for 1 minute, supporting efficient and rapid PCR workflows.

Balanced amplification performance

System 1

System 2

Demonstrates stable and well-shaped amplification curves in multiplex PCR assays, supporting consistent multi-target detection.

Improved premix stability

Pre-mixed System1

When premixed with primers and probes and stored at 37°C for 7 days, no significant performance difference was observed compared with samples stored at -20°C under recommended conditions.