Blood Sample Release Reagent (For Direct LAMP Amplification)

Blood Sample Release Reagent is a sample preparation solution designed for molecular POCT (Point-of-Care Testing) workflows. It enables direct amplification from crude blood lysates without nucleic acid extraction, making it ideal for rapid LAMP-based molecular diagnostics. By simplifying sample processing, it significantly shortens turnaround time while maintaining reliable detection performance comparable to conventional extraction-based methods.

This reagent is compatible with blood samples collected in common anticoagulant tubes, including heparin sodium, EDTA, and sodium citrate, and is optimized for use in direct LAMP fluorescent detection systems.

Product Overview

The Blood Sample Release Reagent is developed to support extraction-free nucleic acid amplification in LAMP assays. It lyses blood samples efficiently and releases target nucleic acids directly into a format suitable for amplification, eliminating the need for time-consuming and labor-intensive extraction procedures.

After treatment, the processed sample can be directly added into a LAMP reaction system for rapid detection of target genes. The workflow is designed to improve efficiency in molecular POCT applications, enabling faster decision-making in clinical and field-testing environments.

Key Features

Extraction-Free Sample Preparation

Enables direct amplification from crude blood lysates, removing the need for nucleic acid purification steps and reducing overall workflow complexity.

Rapid Molecular Testing Workflow

Shortens sample-to-result time by allowing immediate downstream LAMP amplification after sample processing.

High Compatibility with Blood Samples

Suitable for blood collected in:

• Heparin sodium tubes

• EDTA tubes

• Sodium citrate tubes

Optimized for LAMP Fluorescent Detection

Designed for compatibility with fluorescence-based LAMP systems that include buffering capacity, ensuring stable amplification performance.

Flexible Sample Processing Ratio

Supports adjustment of reagent-to-sample ratios, such as processing 10 μL blood with ~500 μL reagent, allowing optimization based on assay requirements.

Simplified POCT Workflow

Reduces operator steps and minimizes contamination risk by eliminating nucleic acid extraction procedures.

Applications

The Blood Sample Release Reagent is widely applicable in molecular diagnostics and POCT LAMP workflows, including:

• Infectious disease rapid detection using blood samples

• Point-of-care molecular diagnostics (POCT)

• Field testing and decentralized laboratory settings

• LAMP-based pathogen detection assays

• Rapid screening workflows in clinical laboratories

• Molecular testing in resource-limited environments

Note:

This reagent is suitable for LAMP fluorescent detection systems with buffering capacity. It is not recommended for buffer-free LAMP colorimetric detection systems due to its alkaline formulation.

FAQ

What is Hzymes’ Blood Sample Release Reagent used for?

It is designed for molecular POCT diagnostics to enable direct amplification from crude blood lysates without nucleic acid extraction, significantly reducing sample processing time.

What sample types are compatible with this reagent?

It is compatible with whole blood and can be adapted for other clinical sample types depending on assay optimization.

Can the released lysate be directly used in LAMP or PCR?

Yes. The reagent is optimized for direct downstream amplification in LAMP systems and selected PCR workflows after proper system validation.

Does this reagent affect amplification efficiency?

It is formulated to minimize PCR/LAMP inhibition while maintaining stable target detection performance in crude sample systems.

Is nucleic acid purification still required?

No purification is required for routine use, which makes it suitable for rapid POCT workflows.