Product Overview

Multiplex qPCR Master Mix (ONE TUBE) is a probe-based multiplex real-time PCR master mix designed for quantitative detection of DNA templates. The formulation incorporates an upgraded Taq DNA polymerase with broad template compatibility and a built-in UDG/dUTP contamination control system to reduce the risk of carryover contamination from previous PCR amplifications.

It’s convenient one-tube format minimizes pipetting steps by requiring only the addition of primers, probes, template, and water, helping simplify workflow and improve operational consistency. The master mix is compatible with multiplex qPCR assay development and supports both standard and fast cycling protocols.

Product Components

Key Features

One-Tube Workflow

• Simplified reaction setup with a ready-to-use 2× master mix.

• Reduces hands-on time and minimizes pipetting errors.

• Suitable for routine qPCR and multiplex assay workflows.

Multiplex Detection Capability

• Designed for probe-based multiplex qPCR applications.

• Supports simultaneous detection of multiple targets in a single reaction.

UDG-Based Contamination Prevention

• Integrated UDG system helps eliminate carryover contamination from previous PCR products.

• Improves assay reliability and reduces false-positive risks.

Broad Template Compatibility

• Optimized Taq DNA polymerase enables reliable amplification across diverse DNA templates.

• Suitable for quantitative DNA detection workflows.

Supports Fast qPCR Protocols

• Compatible with both standard and accelerated cycling conditions.

• Enables rapid amplification and detection while maintaining assay performance.

Applications

Molecular Diagnostic Assay Development

For development and optimization of probe-based multiplex qPCR assays targeting DNA templates.

Pathogen Nucleic Acid Detection

Suitable for DNA-based pathogen detection workflows utilizing real-time PCR technology.

Research Laboratories

Applicable to gene detection, target quantification, and multiplex PCR studies in academic and industrial research settings.

qPCR Kit Manufacturing

Suitable for manufacturers developing multiplex fluorescent qPCR detection kits and related molecular diagnostic products.

Performance Data

Rapid Detection Capability

Using HMD3815A, detection is completed in 15 minutes under the standard protocol. Fast Protocol 95°C 30s, (95°C 1s, 58°C 4s)40 cycles

FAQ

Q1: Does HMD3822 support multiplex detection with complex samples?

A1: Yes. HMD3822 is optimized for multiplex qPCR detection and provides robust amplification performance for samples containing potential PCR inhibitors.

Q2: What sample types are recommended for HMD3822?

A2: HMD3822 is compatible with extracted nucleic acid samples and can support challenging sample types when combined with suitable sample preparation workflows.

Q3: Which resistant RNA MIX and DNA MIX are available, and what types of samples can they tolerate?

A3: The recommended resistant RNA MIX is HMD6906, which supports direct amplification of swab and stool samples, as well as extracted samples from swabs, blood and diarrhea specimens.

The recommended resistant DNA MIX includes HMD3808, HMD6801 and HMD3819, which support direct amplification of samples such as swabs, blood and sputum.