Product Overview

WS Bst DNA Polymerase V2 (Glycerol-Free) is a hot-start version of Bst DNA Polymerase V2, engineered from Bacillus stearothermophilus DNA Polymerase I. The enzyme possesses strong 5'→3' DNA polymerase activity and strand displacement activity, while lacking 5'→3' exonuclease activity, making it ideal for LAMP (Loop-Mediated Isothermal Amplification), RT-LAMP, and other isothermal amplification applications.

Using aptamer-based reversible blocking technology, WS Bst DNA Polymerase V2 remains inactive at lower temperatures, reducing non-specific amplification and false-positive signals during reaction setup. At the reaction temperature, the aptamer dissociates and full enzymatic activity is restored without requiring a separate activation step.

The glycerol-free formulation is specifically designed for the development of lyophilized molecular diagnostic reagents, offering excellent stability and compatibility for freeze-dried LAMP and RT-LAMP assay development.

Key Features

Fast Amplification Performance

Optimized enzyme engineering enables rapid target amplification in LAMP and RT-LAMP assays, helping shorten time-to-result for molecular diagnostics.

Aptamer-Based Warm Start Technology

Reversible aptamer blocking suppresses enzyme activity during reaction setup, minimizing non-specific amplification and improving assay specificity.

High Specificity and Reduced False Positives

Low-temperature enzyme inhibition effectively prevents premature amplification, while full activity is restored at the reaction temperature without an additional activation step.

Excellent Inhibitor Tolerance

Maintains robust amplification performance in the presence of common inhibitors such as guanidine hydrochloride, guanidine isothiocyanate, and potassium chloride, making it suitable for direct amplification workflows.

Glycerol-Free for Lyophilized Assay Development

The glycerol-free formulation facilitates freeze-dried reagent development and improves compatibility with lyophilization processes.

Outstanding Stability

Retains activity after accelerated storage testing and multiple freeze-thaw cycles, supporting reliable manufacturing and long-term reagent performance.

Applications

• Loop-Mediated Isothermal Amplification (LAMP)

• Reverse Transcription LAMP (RT-LAMP)

• Molecular Diagnostic Assay Development

• Pathogen Detection

• Point-of-Care Testing (POCT)

• Direct Amplification Workflows

• Freeze-Dried (Lyophilized) Diagnostic Reagents

• DNA Strand Displacement Reactions

• Rapid Nucleic Acid Detection

Performance Data

Fast Amplification Performance

Polymerization speed is a crucial indicator for evaluating Bst DNA polymerase. The SARS-CoV-2 N gene target (0. 5 cp/μL, 1 cp/μL, and 5 cp/μL) can be successfully detected using WS Bst DNA polymerase V2 (HMD7006). The detection process is faster than Company N at the same template concentration (5 cp/μL).

Aptamer-Based Warm Start Improves Specificity

Hzymes Biotech's WS Bst DNA polymerase V2 (HMD7006 and HMD7007) uses aptamer-based blocking technology. It blocks the enzyme's activity through the tight bond of the aptamer and the polymerase at a lower temperature. But at the working temperature, the binding of the aptamer to the enzyme is released, and the enzyme becomes active.

Example: NTC diagram when detecting SARS-CoV-2 N gene target

Enhanced RT-LAMP Specificity

After aptamer modification, WS Bst DNA polymerase V2 (HMD7006 and HMD7007) has lower enzymatic activity than Company N at 30°C and higher blocking efficiency (More beneficial to prevent non-specific amplification). At the working temperature (65°C), the activity is released and is higher than that of Company N. In an RT-LAMP reaction, NTC does not appear within 60 minutes.

High Inhibitor Tolerance

Hzymes Biotech's Bst DNA polymerase V2 (HMD7005) is compared with competing products in different inhibitory environments (guanidine hydrochloride, guanidine isothiocyanate, KCl, etc.). Hzymes Biotech's Bst DNA polymerase V2 (HMD7005) is more robust than the competitive products and more suitable for different application scenarios, such as direct amplification. It is easier for the subsequent development of LAMP/RT-LAMP reagent.

Robust Performance for Direct Amplification

Bst DNA polymerase V2 and WS Bst DNA polymerase V2 products maintain the same activity after incubation at 37°C for 0, 3, and 7 days. To ensure the development of LAMP/RT-LAMP lyophilized reagents, the activity of glycerol-free Bst DNA polymerase V2 series (HMD7005, HMD7006, HMD7007) remains unchanged after repeated freeze-thaw cycles 40 times.

FAQ

Q1: What is the difference between WS Bst V2 and standard Bst V2?

A: WS Bst V2 is a glycerol-free formulation designed for lyophilization and dry reagent applications.

Q2: Why is glycerol-free important?

A: It improves freeze-drying performance and stability in room-temperature diagnostic formats.

Q3: Is the enzymatic performance the same as standard Bst V2?

A: Yes. It retains the same amplification performance while improving compatibility with dry formats.

Q4: What applications is it suitable for?

A: It is ideal for lyophilized LAMP kits, POCT cartridges, and rapid diagnostic formats.