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Home · Products · Products · Biochemical Diagnostics · Biochemical Diagnostic Enzymes · α-Amylase · α-Glucosidase ·
α-Glucosidase
α-Glucosidase

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3 Ku
HH2002-01
50 Ku
HH2002-02
500 Ku
HH2002-03
1000 Ku
HH2002-04

Cat. No: HH2002 

Activity: ≥40 U/mg 

Storage stability: At least one year at -25 ～ -15 ℃.

Technical Support

Specification


Appearance White amorphous powder, lyophilized
Protein purity ≥90% (from SDS-PAGE)
Activity ≥40 U/mg solid
α-amylase ≤0. 00001%



Properties


ECnumber 3. 2. 1. 20 ( Recombinantfrom microorganism )
Molecular weight 65 kDa (SDS-PAGE)
Isoelectric point 5. 2
Michaelis  constants 5. 8×10-4M  (p-Nitrophenyl-α-D-glucopyranoside)
Inhibitors SDS, Ag+, Hg2+, Cu2+
Optimum pH 7. 1 Fig. 1
Optimum temperature 50℃ Fig. 2
pHstability pH6. 0-8. 0 (25℃, 16h) Fig. 3
Thermal stability Below 50℃ (pH7. 0, 10min) Fig. 4
Storage stability At least one year at -25～-15℃ Fig. 5
Stabilizers BSA



Applications


This enzyme is useful for diagnostic tests for the determination of α-amylase.



Unit definition 


One unit (U) is defined as the amount of enzyme which produces 1 μmol of PNP per min under the conditions described below.

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