Product Overview

PNGase F is the most efficient enzymatic method to remove nearly all N-glycans from glycoproteins. PNGaseF digests the glycosidic bond between the innermost N-acetylglucosamine and asparagine residues. It cleaves N-glycans attached to glycoproteins, including high-mannose, hybrid, and complex oligosaccharide glycoforms.

Key Features

Efficient N-glycan release enzyme

PNGaseF-Fast enables enzymatic removal of N-linked glycans from glycoproteins by cleaving the bond between the innermost N-acetylglucosamine and asparagine residues. It is applicable to high-mannose, hybrid, and complex N-glycan structures.

Preservation of glycan core structure

After enzymatic digestion, released N-glycans retain their core structures, supporting downstream analytical applications.

Rapid reaction setup

Supports fast deglycosylation workflows with a typical setup time of approximately 10 minutes at 50 °C under recommended conditions.

Glycerol-free formulation

Glycerol-free composition is suitable for applications where glycerol interference should be minimized, such as HPLC and mass spectrometry-based analyses under specific workflow requirements.

High purity

Purity is typically ≥95% as determined by standard analytical methods.

Flexible reaction compatibility

Compatible with both denaturing and non-denaturing digestion conditions depending on experimental needs.

Multiple product formats available

Different formats are available to support diverse application requirements (e.g., HBP003006/ HBP003013 /HBP003005).

Applications

N-glycan release for analytical workflows

Used for enzymatic removal of N-linked glycans in workflows typically performed at 50 °C for downstream structural analysis.

CE and LC/LC-MS-based glycan analysis

Suitable for sample preparation in capillary electrophoresis (CE), HPLC, and LC-MS-based N-glycan profiling applications.

Glycoprotein modification for downstream applications

Applied in workflows requiring removal of N-glycans prior to protein modification or bioconjugation processes, including ADC-related research applications depending on experimental design.

Performance Data

Enzymatic digestion efficiency

FAQ

What components are included in the PNGase F-Fast (Glycerol Free) system?

The system typically includes PNGase F-Fast (glycerol-free formulation) and 5×PNGaseF-Fast buffer, designed for rapid N-linked glycan release workflows.

What is the advantage of the glycerol-free formulation?

The glycerol-free formulation is designed for applications where glycerol may interfere with downstream analysis, such as HPLC and LC-MS-based workflows, under specific experimental conditions.

Can this system be used under both denaturing and non-denaturing conditions?

Yes. PNGase F-Fast (Glycerol Free) is compatible with both denaturing and non-denaturing digestion conditions, depending on sample type and experimental requirements.

What are the recommended reaction conditions?

The system supports rapid deglycosylation workflows, with a typical reaction setup of approximately 10 minutes at 50 °C, followed by incubation according to the specific protocol and sample characteristics.

Can reaction conditions be optimized?

Yes. Enzyme amount, incubation time, and reaction conditions can be adjusted based on glycoprotein type, glycan complexity, and downstream analytical needs.

Protocols under denaturing and non-denaturing conditions