Creatinine Amidohydrolase

Product Overview

Creatinine enzyme (EC 3.5.2.10) is a homohexamer with a metal-ion-containing active site, specifically catalyzing the hydrolysis of creatinine to form creatine. It is produced via microbial recombinant expression followed by multi-step chromatographic purification, offering high substrate specificity and controllable residual contaminant levels.

With ultra-large-scale, stable supply capabilities, it helps overcome bottlenecks in raw material procurement. This product is specially designed for global renal-disease in-vitro diagnostic creatinine enzymatic assay systems, exhibiting broad pH tolerance from 5.5 to 10.0 and excellent thermal stability at 65℃.

Its dry-powder specific activity is ≥400 U/mg, and protein purity is 90%. Compared with naturally extracted enzymes, it demonstrates superior batch-to-batch consistency and lower background interference, making it well suited for two-step, anti-interference creatinine assays.

It is applicable to enzymatic quantitative determination of creatinine in serum and urine, automated biochemical reagent formulation, and the development of dry-chemistry solid-phase diagnostic substrates, delivering performance comparable to leading international brands.

Key Features

Excellent Thermal Stability and Extended Shelf Life

HH0106 exhibits excellent thermal stability and long shelf life ensuring reliable stability in the development of creatinine reagents and dry test strips.

Wide pH Stability Range for Broad System Compatibility

Wider pH stability range. It exhibits excellent stability under extreme pH conditions and is compatible with a wider range of reagent systems and application scenarios.

Applications

Core applications: Hydrolyzed creatinine, compatible with CR (catalog no. HH0203), SOX (catalog no. HH0301), and CAT (catalog no. HH2901), for the development and large-scale preparation of enzymatic creatinine reagents.

Reaction Principle

One unit of enzyme activity is defined as the amount of enzyme required to catalyze the formation of 1 μmol of creatine per minute under conditions of pH 6.5 and 37℃.

Specification

CAS	9025-13-2
EC Number	EC 3.5.2.10
Appearance	White amorphous lyophilized powder
Dry powder specific activity	≥400 U/mg
Protein Purity	≥90.0%（SDS-PAGE）
Contaminants	Catalase≤0.1%；ATPase≤0.001%；Proteases≤0.001%；Creatine amidinohydrolase≤0.001%
Protectants	Carbohydrates and BSA
Optimal pH	7.0-8.0
pH stability	pH5.5-10.0（25℃，16h）
Optimal temperature	65℃
Temperature stability	Below 65℃（pH8.0，30min）
Long-term stability	Store at -25 to -15°C for 12 months, maintaining over 90% activity.
Km	5.0×10^-2 M (Creatinine), 8.0×10^-2 M (Creatine)
MW	29kDa（SDS-PAGE）
pI	5.3
Inhibitors	Hg²⁺，Cu²⁺，Fe³⁺

Available Formats

1.HH0106-01 10kU

2.HH0106-02 100kU

3.HH0106-03 1000kU

4.HH0106-04 10000kU

5.HH0106-99 Customizable

Performance Data

Wide Linear Range (20–8,840 μmol/L)

The creatinine reagent exhibits a wide linear range, spanning 20 to 8,840 μmol/L.

Parameter	Specification
Methodology	Enzymatic (Sarcosine oxidase) method
Sample Type	Serum / Plasma/Urine
Reagent	Double Reagents
Assay	2-point
Reagent blank Abs	≤ 0.05
Linearity Range	20~8840 μmol/L, R² ≥0.999
Correlation with Reference reagents	R² = 0.999
Intra-assay Precision	CV ≤3%
Inter-assay Precision	Bias ≤5%
On-board Stability	30 days at 2–8°C
Simulated accelerated stability	14 days at 37°C

Strong Resistance to Drug Interference

Special anti-drug interference. The maximum concentration of calcium dobesilate and etamsylate can reach 20mg/L.

Calcium dobesilate concentration (mg/L)	Low-value samples (μmol/L)	Middle-value samples (μmol/L)	High-value samples (μmol/L)
0	72.2	115.5	217.3
10	71.8	112.6	211.5
20	70.8	110.6	202.8
Relative deviation	0%	-2%	-3%
Relative deviation	-2%	-4%	-7%

Etamsylate concentration (mg/L)	Low-value samples (μmol/L)	Middle-value samples (μmol/L)	High-value samples (μmol/L)
0	71.1	114.8	218.3
10	72.0	113.0	210.7
20	69.9	109.8	206.2
Relative deviation	1.3%	-1.6%	-3.5%
Relative deviation	-1.7%	-4.4%	-5.5%