Product Overview

RNase contamination is one of the most critical challenges in molecular diagnostics workflows, especially in RNA-based detection systems such as RT-PCR and RT-qPCR. RNases are highly stable enzymes widely present in the environment and can rapidly degrade RNA, compromising assay sensitivity and data reliability.

Hzymes GMP-Grade Murine RNase Inhibitor is a recombinant protein expressed in E. coli, designed to protect RNA integrity by specifically binding to RNase A, B, and C in a 1:1 ratio through non-covalent interaction, thereby effectively blocking RNase activity.

Compared with human or porcine RNase inhibitors, the murine version exhibits enhanced resistance to oxidative inactivation, making it particularly suitable for molecular diagnostic reactions that require low DTT concentrations or DTT-sensitive conditions, such as real-time RT-PCR.

This product is manufactured under GMP-grade quality standards with strict impurity control, ensuring low levels of host cell DNA, host cell protein, endotoxin, and nuclease contamination.

Key Features

High RNase Inhibition Efficiency

Effectively inhibits RNase A, B, and C with strong binding activity, ensuring RNA integrity in sensitive diagnostic workflows.

Superior Stability

Maintains full enzymatic activity after repeated freeze-thaw cycles and short-term thermal stress, supporting robust laboratory handling.

Oxidation-Resistant Design

Improved resistance to oxidative inactivation enables stable performance under low-DTT or DTT-sensitive reaction conditions.

GMP-Grade & High Purity

Manufactured under GMP-grade standards with strict QC for HCD, HCP, endotoxin, metal ions, and nuclease residues.

Broad Compatibility in Molecular Diagnostics

Compatible with common reverse transcriptases and DNA polymerases used in RT-PCR and RT-qPCR systems.

Applications

• RT-PCR and RT-qPCR assays

• RNA-based molecular diagnostics workflows

• Clinical RNA sample protection during reverse transcription

• RNA handling and processing in diagnostic reagent systems

• Laboratory workflows requiring high RNA integrity preservation

Performance Data

Freeze-Thaw Stability Test

Hzymes Murine RNase Inhibitor maintains full RNase inhibitory activity after 10 freeze-thaw cycles, demonstrating excellent formulation stability for routine laboratory use.

Thermal Stability Test

No significant loss of activity observed after incubation at 55°C for 5 minutes, indicating strong short-term thermal resistance during reaction setup and handling.

Functional Performance

Efficiently protects RNA from degradation in RT-PCR and RT-qPCR systems by inhibiting RNase A, B, and C activity through stable 1:1 complex formation.

FAQ

Q1: What is the function of RNase Inhibitor?

A: It protects RNA from degradation by binding and inhibiting RNase A-type enzymes.

Q2: What enzymes is it compatible with?

A: It is commonly used with reverse transcriptases in RT-PCR, RT-LAMP, and RNA handling workflows.

Q3: Does it affect reverse transcription efficiency?

A: No. It improves RNA integrity and supports higher cDNA yield indirectly.

Q4: What is its typical application?

A: RT-PCR, RT-qPCR, RNA sequencing workflows, and molecular diagnostics.

Q5: Does it inhibit all RNases?

A: It mainly inhibits RNase A, B, and C, but not all RNase types.