RNase inhibitor is a recombinant murine RNase inhibitor expressed and purified from E.coli. It binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the three enzymes and protecting RNA from degradation. But it is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus.
RNase inhibitor has been tested by RT-PCR, RT-qPCR and IVT mRNA, indicating compatibility with various commercial reverse transcriptases, DNA polymerase, and RNA polymerase.
Compared to human RNase inhibitors, the murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation and thus has higher oxidation resistance, making it stable at low concentrations of DTT (less than 1 mM). The property makes it suitable for reactions where high concentrations of DTT cannot be added (e.g. Real-time RT-PCR).
| Product Name | Cat. No | Size | Volume |
| RNase Inhibitor (Glycerol-free) (80U/μL) | HMD3901A-01 | 2KU | 25μL |
| HMD3901A-02 | 20KU | 250μL | |
| HMD3901A-03 | 200KU | 2500μL |
Storage
Store at -25～-15℃;Freeze-thaw cycles ≤ 5 times;
Valid for 1 years;
