Product Description
Co-capping T7 in vitro transcription reagent is a
co-transcription capping reagent for in vitro
transcription of 5'capped mRNA to produce Cap-1 structure with an optimized RNA
synthesis formulation. The kit is capable of synthesizing mRNAs that contain a natural
Cap-1 structure in a single simplified reaction without compromising RNA yield using linear double-stranded DNA containing T7 promoter
followed by an AG initiation sequence. The kit contains modified nucleotides (N1-Me-pUTP) as alternative. Capped mRNAs with modified
nucleotide have lower immunogenic potential.
Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size
can be scaled up to yield milligram-level RNA
as needed.
RNA synthesized from the kit is suitable for many applications including RNA structure and
function studies, transfections, microinjections, in vitro translation, preclinical mRNA
therapeutic mRNA studies.
Product Information
Product Name | Cat. No |
Co-capping T7 in vitro transcription reagent (N1-Me-pUTP, CAP GAG (3’OMe)) | HBP001509 |
Transportation and storage
Transportation under 0°C and storage at -25~ -15°C.