Product Description
DNase I (Deoxyribonuclease I) is an endodeoxyribonuclease that can digest single- or double- stranded DNA. It recognizes and cleaves phosphodiester bonds to produce monodeoxynucleotides or single- or double- stranded oligodeoxynucleotides with phosphate groups at the 5'-terminal and hydroxyl at the 3'-terminal. The activity of DNase I depends on Ca 2+ and can be activated by divalent metal ions such as Mn 2+ and Zn 2+ . 5 mM Ca 2+ protects the enzyme from hydrolysis. In the presence of Mg 2+ , the enzyme could randomly recognize and cleave any site on any strand of DNA. In the presence of Mn 2+ , the double strands of DNA can be simultaneously recognized and cleaved at almost the same site to form flat end DNA fragments or sticky end DNA fragments with 1-2 nucleotides protruding.
Product Information
| Product Name | Cat. No | Size |
| DNase I | HBP000910-1 | 20 μL/0.1 KU |
| HBP000910-3 | 1 mL/5 KU | |
| HBP000910-4 | 10 mL/50 KU | |
| HBP000910-5 | 100 μL/0.5 KU |
Transportation and Storage
Storage Stability: -25℃~-15℃ for storage; Transport Stability: Transport under ice packs; Supplied in: 10 mM Tris-HCl, 2 mM CaCl2, 50% glycerol, pH 7.6 @25℃.
Unit Definition
One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C.
