Diaphorase

Protease K |Creatinase lamp |dNTP |All-purpose nuclease mRNA

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Diaphorase

CAS：9001-18-7

5 Ku: HH2201-01

100 Ku: HH2201-02

500 Ku: HH2201-03

1000 Ku: HH2201-04

Cat. No: HH2201

Activity: ≥600. 0 U/mg

Storage stability: At least one year at -25～-15 ℃

Technical Support

Enzymatic Performance Evaluation

Advantage 1: Excellent Thermal Stability and Batch Consistency

Reduces the complexity of reagent development.

Hzymes' product retains over 90% activity after 7-day incubation at 42°C, with a half-life exceeding 300 minutes at 75°C. It also demonstrates strong batch-to-batch consistency.

Advantage 2: Broad Stability Range in Temperature and pH

Suitable for a wide variety of reagent systems and application scenarios.

Hzymes’ product maintains stable enzymatic activity below 70°C and within the pH range of 4. 5 to 10.

Test Parameters	Hzymes	Brand T	Brand A
Specific Activity of Enzyme Powder (U/mg)	818	841	39
Thermal Stability	≤70℃	≤30℃	≤50℃
pH Stability	4. 5~10. 0	7. 5~9. 0	6. 0~9. 0
Optimal Temperature (°C)	45	50	50
Optimal pH	7. 5	8. 5	8
Half-Life at 75°C (min)	390. min ( 75℃ )	30min ( 70℃ )	10min ( 55℃ )

Preparation and specification

Appearance	Yellow amorphous powder, lyophilized
Purity (SDS-PAGE)	≥90%
Enzyme powder specific activity	≥600. 0 U/mg
Glucose-6-phosphate dehydrogenase	≤0. 01%
NADH/NADPH oxidase	≤0. 01%

Properties

EC number	1. 6. 5. 2 (Recombinant from microorganism)
Molecular weight	28 kDa (SDS-PAGE)
Isoelectric point	5. 2
Michaelis constants	7. 4 ×10^-4 M (NADH), 6. 0×10^-2 M (NADPH)
Inhibitors	Fe³⁺, Mn²⁺, Cu²⁺
Optimum pH	7. 5	Fig. 1
Optimum temperature	45℃	Fig. 2
pH stability	pH 4. 5-9. 0 (25 ℃, 16 h)	Fig. 3
Thermal stability	Below 50 ℃ (pH 7. 5, 30 min)	Fig. 4
Storage stability	At least one year at -25～-15 ℃	Fig. 5
Stabilizers	FMN, NAD (P)H

Applications

This enzyme is useful for colorimetric determination of NAD (P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD (P)H.

Unit definition

One unit (U) is defined as the amount of enzyme which consumes 1 μmol of DCPIP per min under the conditions described below.

Related Products

Fructosyl-Peptide Oxidase

English name: Fructosyl-peptide oxidase

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Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.

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Service Hotline: +86 400-808-5320

Large-scale production base: Building 6, Precision Medical Industry Base, Wuhan, China.

Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.

Global Marketing Center: Hzymes Building, Fengxian District, Shanghai, China.

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