Product Overview

RT-LAMP Probe Master Mix (In situ Lyophilization) is a ready-to-use reagent designed for rapid isothermal amplification of DNA and RNA targets. The system integrates Bst DNA polymerase, a heat-stable reverse transcriptase, reaction buffer, dNTPs, Mg²⁺, and optimized lyophilized protective components into a single formulation.

Using an in situ lyophilization process, the master mix is directly freeze-dried within the reaction system, ensuring uniform reagent distribution and enhanced batch-to-batch consistency. This format improves enzyme stability and simplifies downstream assay preparation.

For use, only the primer/probe mix and nucleic acid template need to be added, making the workflow highly efficient and suitable for molecular diagnostics and POCT applications.

The product is supplied in a stable lyophilized powder form and can be stored and transported at 2–8°C for up to 12 months, supporting reliable performance in both laboratory and field environments.

Product components

Key Features

Integrated RT-LAMP One-Step System

Combines reverse transcription and LAMP amplification in a single reaction, enabling detection of both RNA and DNA targets.

In Situ Lyophilization Technology

Enzymes and reagents are freeze-dried directly in the reaction system, ensuring uniform composition, improved stability, and consistent performance.

Ready-to-Use Format

Only primer/probe mix and template are required for reaction setup, significantly reducing preparation time and pipetting steps.

High Sensitivity and Fast Amplification

Optimized formulation with Bst DNA polymerase and thermostable reverse transcriptase enables rapid nucleic acid amplification with strong strand displacement activity.

Excellent Stability and Storage Convenience

Stable for 12 months at 2–8°C, supporting simplified logistics and reduced cold-chain dependency.

Applications

RT-LAMP Probe Master Mix (In situ Lyophilization) is widely used in molecular diagnostic workflows, including:

• RNA pathogen detection (viral RNA identification)

• DNA pathogen detection (bacterial and viral detection)

• Isothermal amplification-based molecular diagnostics

• Point-of-care testing (POCT) platforms

• Rapid infectious disease screening

• Field-deployable diagnostic assays

• Kit development and molecular assay manufacturing

FAQ

Q1: What is in situ lyophilization in this product?

A: In situ lyophilization means the reagents are freeze-dried directly within the reaction system, ensuring uniform distribution and improved consistency.

Q2: What are the benefits of in situ lyophilization compared to conventional lyophilization?

A: Compared to standard lyophilization, in situ lyophilization provides:

• More uniform reagent distribution

• Improved batch-to-batch consistency

• Enhanced stability of enzyme activity

• Better performance reproducibility

Q3: What do users need to add before use?

A: Only primer/probe mix and template nucleic acid are required for reaction setup.

Q4: Can this product detect both RNA and DNA targets?

A: Yes. The integrated RT enzyme and Bst polymerase system enable dual RNA/DNA detection in a single reaction.

Q5: What is the storage condition and shelf life?

A: Store at 2–8°C, with a shelf life of 12 months.

Q6: What applications is this product suitable for?

A: It is widely used in:

• POCT molecular diagnostics

• Infectious disease detection

• Field-deployable testing systems

• Commercial diagnostic kit development