Dual-Block Taq antibody V1 is a product developed by our company that targets and blocks both the 5’→3’ DNA polymerase activity and the 5’→3’ exonuclease activity of Taq DNA polymerase at two separate sites. During the temperature rise in the PCR pre-denaturation, Dual-Block Taq antibody V1 effectively prevents the leakage of Taq DNA polymerase activity and inhibits the 5’→3’ polymerase activity, thereby suppressing non-specific amplification caused by primers under low temperature conditions. It also inhibits the 5’→3’ exonuclease activity, preventing the probes or primers from being cleaved and affecting the performance of the reagents. This product can fully release the activity of Taq DNA polymerase after heating at 95°C for only 30 seconds. Using this product to block Taq DNA polymerase significantly improves amplification specificity and the detection rate of low templates. No special inactivation treatment is required for the antibody when using this product, making it compatible with existing PCR reaction conditions.
Product Information
| Product Name | Cat. No | Size |
| Dual-Block Taq antibody V1 （5 mg/mL） |
HMD0311-01 | 0.5 mg |
| HMD0311-02 | 5 mg | |
| HMD0311-03 | 50 mg |
Precaution
• Avoid repeated freeze-thaw cycles and wear gloves during operation.
• Due to differences in the specific activity of different Taq DNA polymerase, the blocking ratio can be adjusted appropriately, with a blocking efficiency of over 95% being preferable.
