Product Overview
One-Step Multiplex RT-qPCR Master Mix (ONE TUBE) is a ready-to-use real-time PCR reagent designed for primer-probe premixing workflows. It enables a simplified one-tube setup where primers and probes can be pre-mixed in advance, and only sample nucleic acids need to be added for downstream detection.
The system supports both singleplex and multiplex RT-qPCR detection and integrates a UDG-dUTP carryover contamination prevention system, effectively reducing the risk of aerosol contamination and ensuring reliable amplification results.
This master mix is suitable for high-throughput and routine molecular diagnostic workflows requiring speed, stability, and operational convenience.
Key Features
One-Tube Workflow Design
Supports primer and probe premixing, reducing pipetting steps and simplifying experimental setup.
Multiplex Compatibility
Enables simultaneous detection of multiple targets in a single reaction without compromising assay design flexibility.
Carryover Contamination Control
Integrated UDG-dUTP system effectively prevents amplification from contaminated PCR products.
Rapid Amplification System
Optimized for fast RT-qPCR protocols to shorten overall detection workflow.
Operational Convenience
Pre-formulated master mix improves workflow consistency and reduces hands-on time.
Applications
One-Step Multiplex RT-qPCR Master Mix (ONE TUBE) is suitable for molecular diagnostic applications based on real-time RT-qPCR technology, including:
• Respiratory pathogen detection (e.g., Influenza A/B, RSV, and related viruses)
• Enteric pathogen nucleic acid detection
• Hepatitis virus detection and screening workflows
• Multiplex infectious disease panel development
• IVD kit manufacturing and assay development
• Clinical molecular diagnostics and research laboratories
Performance Data
Accelerated Stability Validation
Influenza A/B & RSV 4-plex system maintained stable performance after 10 days at 37°C under accelerated stability testing conditions.
Multiplex Assay Demonstration
Successful detection of metapneumovirus, Mycoplasma pneumoniae, and parainfluenza virus type 1 in multiplex formats, confirming assay robustness across diverse targets.
Fast Workflow Verification
Fast RT-qPCR protocol achieved approximately 30-minute total detection time while maintaining reliable amplification efficiency.
Low Copy Detection Capability
Demonstrated stable detection of single-copy nucleic acid templates in controlled experimental conditions.
FAQ
Q1: What types of samples can be detected using HMD3913?
A1: HMD3913 is designed for one-step multiplex RT-qPCR detection and supports RNA analysis from various sample types, including extracted samples and inhibitor-containing clinical specimens.
Q2: Can HMD3913 be used for direct amplification of RNA samples?
A2: Yes. HMD3913 provides strong resistance to common inhibitors and is suitable for direct amplification workflows with properly processed samples.
Q3: Which resistant RNA MIX and DNA MIX are available, and what types of samples can they tolerate?
A3: The recommended resistant RNA MIX is HMD6906, which supports direct amplification of swab and stool samples, as well as extracted samples from swabs, blood and diarrhea specimens.
The recommended resistant DNA MIX includes HMD3808, HMD6801 and HMD3819, which support direct amplification of samples such as swabs, blood and sputum.
