Preparation and specification
| Appearance | Colorless transparent liquid |
| Protein purity | ≥90% (from SDS-PAGE) |
| Activity | ≥5 U/mL |
| Β-N-acetylglucosaminidase | ≤ 0.2% |
| a-mannosidase | ≤ 0.05% |
| β-galactosidase | ≤ 0.1% |
Properties
| EC number | 3.2.1.51(Recombinantfrom microorganism) | |
| Molecular weight | 55 kDa (SDS-PAGE) | |
| Isoelectric point | 6.2 | |
| Michaelis constants | 7.5 | Fig.1 |
| Optimum pH | 80℃ | Fig.2 |
| Optimum temperature | pH7.0-10.0(25℃,16h) | Fig.3 |
| pH stability | Below90℃(pH8.0,30min) | Fig.4 |
| Thermal stability | At least one year at-25～-15℃ | Fig.5 |
Applications
This enzyme is used in the reagent calibration of α-L-fucosidase and research of quality control products.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of CNP per min under the conditions described below.
