Product Overview

α-L-fucosidase (AFU) is recombinantly expressed from thermophilic deep-sea microorganisms, serving as a highly pure, assay-specific calibrator and quality-control material for the diagnosis of liver diseases. It is paired with the specific substrate CNP-AFU (HH4701) to enable quantitative clinical measurement of AFU enzymatic activity. Compared with traditionally tissue-derived AFU, this product exhibits exceptional thermal stability: its optimal reaction temperature is 80°C, with a half-life of up to 214 minutes at 90°C; enzyme activity remains unchanged after prolonged incubation at 37°C. With a molecular weight of 55 kDa, it offers higher purity and lower impurity levels, maintains stable activity across a pH range of 7.0-10.0, and is compatible with a wide variety of buffer systems. Moreover, produced via large-scale microbial fermentation, it is not constrained by tissue or organ sources, allowing for reliable, high-volume supply. The enzyme solution can be stored sealed at -20°C for 12 months, retaining over 90% of its activity, and a lyophilized powder formulation is also available for even longer shelf life. It demonstrates excellent chemical compatibility across diverse buffer systems, and its linear calibration curve shows outstanding agreement, ensuring precise matching with AFU assays. This high-purity AFU is also suitable for glycan-based identification and research applications.

Key Features

Outstanding thermal stability

Derived from thermophilic microorganisms found in the deep sea, this enzyme exhibits exceptional thermal stability, with a half-life of 214 minutes at 90°C. Its optimal temperature is 80°C, and its activity remains unchanged after incubation at 37°C for 10 days.

High compatibility and adaptability

Resistant to chemicals such as EDTA, NaN₃, Proclin 300, and Triton X-100. Activity remains stable across a pH range of 7.0-10.0, making it compatible with various buffer systems. It also demonstrates excellent dilution linearity, with performance closely aligned with AFU reagents.

Long-term stability

Enzyme solutions stored sealed at -20°C retain over 90% of their activity for up to 12 months. Lyophilized powder is also available for even longer shelf life.

Thermal Stability

30 min-treatment with 50 mM Tris-HCl buffer, pH 8.0.

Enzyme concentration: 1 mg/mL

Optimal temperature

25°C, 16 h-treatment with 50 mM buffer solution: pH 4.5-5.5, Acetate; pH 6.0-8.0, K-phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH.

Enzyme concentration: 1 mg/mL

Applications

Liver Function - AFU Assay

Serves as a calibrator and quality control material for AFU assay kits, with the accompanying specific substrate CNP-AFU (HH4701) used in the development and large-scale preparation of AFU assay reagents.

Glycobiology Research

Identification and Detection of Fucose Modifications.

Reaction Principle

Unit enzyme activity is defined as the amount of enzyme required to hydrolyze 1 μmol of CNP-AFU per minute under conditions of pH 8.0 and 37°C.

Specification

Available Formats

• HH4602-01 1kU

• HH4602-02 5kU

• HH4602-99 Customizable

Performance Data

Long-Term Stability

The liquid enzyme retains over 90% activity after 12 months of sealed storage at -20°C. A lyophilized formulation with extended shelf life is also available.

Storage stability (-25 to -15°C)