Specification
| Appearance | White amorphous powder, lyophilized |
| Protein purity | ≥90% (from SDS-PAGE) |
| Activity | ≥40 U/mg solid |
| α-amylase | ≤0.00001% |
Properties
| ECnumber | 3.2.1.20(Recombinantfrom microorganism) | |
| Molecular weight | 65 kDa (SDS-PAGE) | |
| Isoelectric point | 5.2 | |
| Michaelis constants | 5.8×10-4M (p-Nitrophenyl-α-D-glucopyranoside) | |
| Inhibitors | SDS,Ag+,Hg2+,Cu2+ | |
| Optimum pH | 7.1 | Fig.1 |
| Optimum temperature | 50℃ | Fig.2 |
| pHstability | pH6.0-8.0(25℃,16h) | Fig.3 |
| Thermal stability | Below50℃(pH7.0,10min) | Fig.4 |
| Storage stability | At least one year at -25～-15℃ | Fig.5 |
| Stabilizers | BSA | |
Applications
This enzyme is useful for diagnostic tests for the determination of α-amylase.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of PNP per min under the conditions described below
