Product Description
Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active site catalytic triad (Asp 39-His 69-Ser 224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
Product Information
| Product Name | Cat. No | Size |
| Proteinase K (liquid) | HBP800001 | 1ml |
| HBP800002 | 10ml |
Specification
| Appearance | Colorlesstolightbrownliquid |
| Activity | ≥800U/ml |
| Proteinconcentration | ≥20mg/ml |
| DNase | None detected |
| RNase | None detected |
| Nickase | None detected |
Properties
| ECnumber | 3.4.21.64(Recombinantfrom Tritirachiumalbum) | |
| Molecularweight | 29kDa(SDS-PAGE) | |
| Isoelectricpoint | 7.81 | |
| OptimumpH | 7.0-12.0 | Fig.1 |
| Optimum temperature | 65℃ | Fig.2 |
| pHstability | pH4.5-12.5(25℃,16h) | Fig.3 |
| Thermalstability | Below50℃(pH8.0,30min) | Fig.4 |
| Storagestability | Over90%activityfor12months at25℃ | Fig.5 |
| Activators | SDS,urea | |
| Inhibitors | Diisopropylfluorophosphate; phenylmethylsulfonylfluoride |
|
| Storagecondition | 2-8℃ | |
Applications
Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and preparation of heparin; Preparation of chromosome DNA by pulsed electrophoresis; Western blot; Enzymatic glycosylated albumin reagents in vitro diagnosis.
Unit definition
One unit (U) is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute under the following conditions.
Precautions
Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction. Cause serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. May cause respiratory irritation.
