Proteinase K (liquid)
Proteinase K (liquid)

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1ml
HBP800001
10ml
HBP800002

Cat. No: HBP800001、HBP800002

Activity: ≥800 U/mL 

Storage condition: Over 90% activity for 12months at 25℃

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Product Description

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active site catalytic triad (Asp 39-His 69-Ser 224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.


Product Information

Product Name Cat. No Size
Proteinase K (liquid) HBP800001 1ml
HBP800002 10ml


Specification

Appearance Colorlesstolightbrownliquid
Activity ≥800U/ml
Proteinconcentration ≥20mg/ml
DNase None detected
RNase None detected
Nickase None detected

Properties

ECnumber 3.4.21.64(Recombinantfrom Tritirachiumalbum)
Molecularweight 29kDa(SDS-PAGE)
Isoelectricpoint 7.81
OptimumpH 7.0-12.0 Fig.1
Optimum temperature 65℃ Fig.2
pHstability pH4.5-12.5(25℃,16h) Fig.3
Thermalstability Below50℃(pH8.0,30min) Fig.4
Storagestability Over90%activityfor12months at25℃ Fig.5
Activators SDS,urea
Inhibitors Diisopropylfluorophosphate;
phenylmethylsulfonylfluoride
Storagecondition 2-8℃


Applications

Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and preparation of heparin; Preparation of chromosome DNA by pulsed electrophoresis; Western blot; Enzymatic glycosylated albumin reagents in vitro diagnosis.


Unit definition 

One unit (U) is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute under the following conditions.


Precautions

Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction. Cause serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. May cause respiratory irritation.

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Service Hotline: +86 400-808-5320

Large-scale production base: Building 6, Precision Medical Industry Base, Wuhan, China

Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.

Global Marketing Center: Hzymes Building, Fengxian District, Shanghai, China.

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Contact Us

Service Hotline: +86 400-808-5320

Large-scale production base: Building 6, Precision Medical Industry Base, Wuhan, China.

Logistics & Supply Chain Center:417 Main St, Little Rock, AR 72201. United States.

Global Marketing Center: Hzymes Building, Fengxian District, Shanghai, China.

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