KEY RECOMMENDATIONS

Benefits

Supports raw material selection with high standards to guarantee product quality and reduce negative values in clinical test results; Maintains superior stability, stable for 18 months if tightly sealed and stored at < 4°C and for 28 days after opening; Displays high intra- and inter-assay precision, with the intra-assay CV ≤5% and the inter-assay CV ≤4%.

Anti-interference Performance

Vitamin C ≤30 mg/dL
Bilirubin ≤50 mg/dL
Glucose ≤1000 mg/dL
Lipemia ≤2000 mg/dL

Applications

In vitro test for the quantitative determination of creatine kinase-MB (CK-MB) activity in serum on photometric systems. Creatine kinase (CK) is an enzyme, which consists of isoenzymes mainly of the muscle (CK-M) and the brain (CK-B). CK exists in serum in dimeric form as CK-MM, CK-MB, and CK-BB and as macroenzyme. Determination of CK-MB values possesses highly specificity in myocardial damages. So, measurement of CK-MB is used for diagnosis and monitoring of myocardial infarction.

Principle

In the reaction, the creatine kinase-B catalyzes the transfer of a phosphate group from the creatine phosphate substrate to ADP. The subsequent formation of ATP is measured through the use of two coupled reactions catalyzed by hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH) which results in the production of the reduced form of NADPH from NADP. The rate of absorbency increase at 405nm is directly proportional to the activity of creatine kinase-B.

Storage and stability

Up to expiration date indicated on the label, when stored unopened at 2-8℃ and protected from light.

 
Once opened, the reagents are stable for 28 days when refrigerated on the analyzer or refrigerator. Contamination of the reagents must be avoided. Do not freeze the reagents.

 
Once dissolved, the calibrator are stable for 7 days at 2–8℃, the control are stable for 7 days at 2–8℃, do not freeze

Warnings and precautions

1. For in vitro diagnostic use.

 
2. Take the necessary precautions for the use of laboratory reagents.

 
3. Preservative contained. Do not swallow. Avoid contact with skin and mucous membranes.

 
4. Disposal of all waste material should be in accordance with local guidelines.
5. Material safety data sheet is available on request for professional users.