Product Overview

LAMP Probe Master Mix (In Situ Lyophilization) is a ready-to-use reagent system designed for rapid and reliable DNA isothermal amplification. The product contains a complete reaction system including reaction buffer, an optimized enzyme mixes of Bst DNA polymerase, and lyophilized protectant components.

The reaction buffer is pre-formulated with Mg²⁺, dNTPs, and other essential components required for efficient LAMP amplification. Unlike conventional liquid formats, this product is prepared using an in situ lyophilization process, where reagents are freeze-dried directly in the reaction vessel, forming a stable powder formulation.

This design ensures improved reagent stability, simplified workflow, and reduced risk of contamination. Users only need to add the primer-probe mix and template DNA to initiate amplification, making it highly suitable for fast molecular diagnostic workflows.

Product components

Key Features

In situ lyophilization technology for enhanced stability

Reagents are freeze-dried directly in the reaction tubes, improving stability and reducing degradation risk during transport and storage.

Ready-to-use LAMP system

Pre-loaded reaction components eliminate the need for complex preparation. Only primers, probes, and template are required for reaction setup.

Optimized Bst DNA polymerase system

High-performance enzyme mix enables efficient strand displacement DNA synthesis for rapid and sensitive isothermal amplification.

Reduced contamination risk

Closed-system lyophilization format minimizes handling steps and reduces the risk of cross-contamination.

High reproducibility and batch consistency

Standardized in situ production ensures uniform reaction performance across batches and strips.

Suitable for molecular diagnostic workflows

Designed for fast and reliable nucleic acid detection in both laboratory and POCT environments.

Applications

LAMP Probe Master Mix (In Situ Lyophilization) is suitable for DNA isothermal amplification-based applications, including:

• Pathogen detection and infectious disease diagnostics

• Point-of-care testing (POCT) molecular workflows

• Clinical nucleic acid detection assays

• Food safety testing and contamination screening

• Veterinary diagnostic applications

• Environmental microbial detection

FAQ

Q1: What is “in situ lyophilization” in this product?

A: In situ lyophilization means the reagents are freeze-dried directly inside the reaction tubes. This creates a pre-loaded dry reaction system that only requires addition of primers and template.

Q2: Does this product contain reverse transcriptase activity?

A: No. It contains Bst DNA polymerase with strong strand displacement activity but does not support RNA reverse transcription.

Q3: What are the advantages of in situ lyophilization compared to bead format?

A: Compared with lyophilized beads, in situ lyophilization offers:

• Stronger anti-contamination protection (closed-tube system)

• More integrated kit format

• Reduced manual handling steps

• Higher suitability for automated or POCT workflows

Q4: Does this product include UDG?

A: Standard products do not include UDG. Customized UDG-integrated versions are available upon request.

Q5: What applications is this format best suited for?

A: It is ideal for:

• Point-of-care molecular diagnostics

• Rapid pathogen detection kits

• Fully integrated diagnostic cartridge systems

• High-throughput standardized testing workflows