Specification
| Appearance | White amorphous powder, lyophilized |
| Enzyme powder specific activity | ≥1300.0U/mg |
| Pyruvatekinase | ≤0.5% |
| Lactic dehydrogenase | ≤0.01% |
| Glutamic oxalate transaminase | ≤0.01% |
Properties
| ECnumber | 1.1.1.37 (Recombinantfrom microorganism) | |
| Molecular weight | 35kDa (SDS-PAGE) | |
| Isoelectric point | 5.61 | |
| Michaelis constants | 7.8×10-5M(Oxaloacetate),1.2×10-4M (NADH) | |
| Inhibitors | Hg2+ | |
| Optimum pH | 8.0-9.0 | Fig.1 |
| Optimum temperature | 60℃ | Fig.2 |
| pH stability | pH 4.0-9.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 50℃ (pH7.5,30min) | Fig.4 |
| Storage stability | At least one year at-25~-15℃ | Fig.5 |
Applications
This enzyme is used in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
Unit definition
One unit (U) is defined as the amount of enzyme which consumes 1 μmol of NADH per min under the conditions described below.
