L-Glycerol-3-Phosphate Oxidas

Product Overview

The six lipid-profile parameters include triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), apolipoprotein A (ApoA), and apolipoprotein B (ApoB). Glycerol-3-phosphate oxidase (G3PO) is the key enzyme in clinical TG assay reagents. This product is an AI-engineered, multi-point-mutated recombinant enzyme that specifically addresses the shortcomings of wild-type enzymes, namely, poor thermal stability and limited formulation compatibility. It exhibits a dry-powder specific activity of ≥12 U/mg, a protein purity of ≥90%, and tightly controlled levels of contaminating enzymes. The enzyme remains stable across a broad pH range of 5.5-9.5, demonstrates excellent tolerance to 45°C conditions, and is compatible with a variety of nonionic surfactants and chelating agents commonly used in lipid-assay formulations. Compared with imported chemically modified enzymes, this product eliminates the need for chemical modification and avoids batch-to-batch variability; its fermentation and purification processes are well-controlled, enabling scalable manufacturing and enhanced batch-to-batch consistency. When paired with LPL (HH1008), GK (HH1207), and peroxidase (POD), it forms a complete four-enzyme coupled TG assay system, offering wide linearity, measurement deviations of <5%, and suitability for both fully automated biochemical analyzers and dry-format lipid-assay development. The reagent also exhibits robust resistance to freeze-thaw cycles and accelerated aging, meeting global IVD raw material procurement standards.

Key Features

Excellent thermal stability, with guaranteed stability of the TG reagent

Stable over a broad pH range of 5.5-9.5

Offering enhanced compatibility with TG reagents and greater flexibility in experimental conditions.

>75% Activity Across pH 4.5-10.0

It exhibits over 75% activity across a broad pH range of 4.5-10.0. With enhanced compatibility and greater flexibility in operational conditions for TG reagents.

Buffer solution: pH 4.5-5.5, acetate; pH 6.0-8.0, phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH.

Enzyme concentration: 1 mg/mL

Broad Chemical Resistance

Resistant to a wide range of chemicals, including various surfactants, metal ions, and chelating agents.

Effect of Various Chemicals on G3PO (37°C 24 h Residual activity)
No.	Chemicals	Concentration	Vendor A	Vendor B	Hzymes
1	MgCl₂	2 mM	94.5%	98.9%	96.9%
2	CaCl₂	2 mM	105.2%	95.8%	94.8%
3	FeSO₄	2 mM	73.7%	70.3%	68.7%
4	FeCl₃	2 mM	0.7%	2.8%	3.8%
5	CoCl₂	2 mM	108.9%	92.6%	78.9%
6	MnCl₂	2 mM	106.9%	88.0%	89.1%
7	ZnCl₂	2 mM	53.8%	0.4%	1.9%
8	NiSO₄	2 mM	80.6%	91.9%	91.4%
9	AgNO₃	2 mM	84.0%	92.2%	94.4%
10	CuSO₄	2 mM	86.7%	1.4%	1.1%
11	NaF	2 mM	99.5%	93.3%	94.0%
12	NaN₃	20 mM	102.4%	93.3%	96.7%
13	EDTA	50 mM	94.8%	91.2%	90.1%
14	Borate	20 mM	102.4%	96.1%	93.7%
15	IAA	2 mM	98.8%	94.0%	94.0%
16	NEM	2 mM	99.1%	88.7%	92.9%
17	Hydroxylamine	2 mM	106.6%	95.8%	94.0%
18	Triton X-100	0.10%	101.9%	94.0%	95.2%
19	Brij 35	0%	101.9%	97.5%	95.9%
20	Tween 20	0.10%	103.8%	96.8%	98.2%
21	Span 80	0.10%	94.3%	95.1%	83.5%
22	Na-cholate	0.10%	104.3%	97.2%	94.4%
23	SDS	0.50%	4.3%	40.4%	0.8%
24	DAC	0.50%	28.0%	0.7%	0.0%

Excellent Storage, Accelerated, and Freeze-Thaw Stability

The enzyme powder exhibits excellent refrigerated storage and accelerated degradation stability, while the enzyme solution demonstrates robust freeze-thaw stability, ensuring consistent quality even under extreme transportation conditions.

Robust Manufacturing with Excellent Batch Consistency

The manufacturing process is robust, with stringent quality control, resulting in minimal batch-to-batch variability in both specific protein activity and specific dry-powder activity.

Applications

Lipid Profile Testing - Triglyceride Assay: The core reagents, G3PO and LPL (HH1008) together with GK (HH1207), are used for the development and large-scale preparation of triglyceride assay kits employing an enzymatic method.

Reaction Principle

One unit of enzyme activity is defined as the amount of enzyme required to catalyze the consumption of 1 μmol of glycerol-3-phosphate per minute under conditions of pH 8.0 and 37°C.

Specification

Parameter	Specification
CAS	9046-28-0
EC Number	EC 1.1.3.21
Appearance	Yellow amorphous lyophilized powder
Dry powder specific activity	≥12 U/mg
Protein Purity	≥90% (SDS-PAGE)
Contaminants	Cholesterol oxidase ≤0.001%; Lactate oxidase ≤0.002%; Uricase ≤0.05%
Optimal pH	8.0-8.5
pH stability	5.5-9.5
Optimal temperature	55°C
Temperature stability	Below 45°C (pH 7.5, 30 min)
Long-term stability	Store at -25 to -15°C for 12 months, maintaining over 90% activity.
MW	75 kDa (SDS-PAGE)
pI	4.2
Inhibitors	Fe³⁺

Available Formats

• HH1103-R2-01 5kU

• HH1103-R2-02 100kU

• HH1103-R2-03 500kU

• HH1103-R2-04 1000kU

• HH1103-R2-99 Customizable

Performance Data

Wide Linear Range & Excellent Reproducibility

The TG reagent exhibits a wide linear range and excellent batch-to-batch reproducibility.

Strong Method Agreement & Long-Term Stability

The TG reagent exhibits minimal deviation in measured values compared to reagents from well-known manufacturers, and demonstrates excellent stability: its accelerated stability at 37°C can last up to 21 days, while its open-vial stability on the instrument reaches one month.

Parameter	Specification
Methodology	Enzymatic GPO-POD method
Sample Type	Serum / Plasma
Reagent	Single Reagent
Assay	1-point
Reagent blank Abs	≤0.1000
Linearity Range	0.10-11.4 mmol/L. R² ≥0.990
Correlation with Reference reagents	R² = 0.999
Intra-assay Precision	CV ≤ 3%
Inter-assay Precision	Bias ≤ 5%
On-board Stability	30 days at 2-8°C
Simulated accelerated stability	21 days at 37°C