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Home · Products · Products · Biochemical Diagnostics · Biochemical Diagnostic Enzymes · Creatine kinase / Creatine Kinase Isoenzyme · Hexokinase ·
Hexokinase
Hexokinase

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  • 230
2 Ku
HH0705-01
10 Ku
HH0705-02
100 Ku
HH0705-03
500 Ku
HH0705-04

Cat. No: HH0705 

Activity: ≥45. 0 U/mg 

Storage stability:   At least one year at -25～-15℃ 

Technical Support

Specification


Appearance White to light yellow amorphous powder, lyophilized
Purity (SDS-PAGE) ≥90%
Enzyme powder specific activity ≥45. 0 U/mg
NADH/NADPH oxidase ≤0. 01%
ATPase ≤0. 03%
Glucose-6-phosphate dehydrogenase ≤0. 01%



Properties


EC number 2. 7. 1. 1 (Recombinant from microorganism)
Molecular weight 36 kDa (SDS-PAGE)
Isoelectric point 5. 64
Michaelis constants 3. 2×10-4M (D-glucose), 8. 3×10-5M (ATP)
Inhibitors SDS, Ag+, Fe3+, Hg2+, Cu2+
Optimum pH 8 Fig. 1
Optimum temperature 45℃ Fig. 2
pH stability pH 6. 5-9. 0 (25℃, 16h) Fig. 3
Thermal stability Below 37℃ (pH 8. 0, 30 min) Fig. 4
Storage stability At least one year at -25～-15℃ Fig. 5
Stabilizers BSA



Applications


This enzyme is useful for enzymatic determination of glucose or creatinine kinase activity when coupled with glucose-6-phosphate dehydrogenase.



Unit definition 


One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADH per min under the conditions described below.

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