Product Overview

Proteinase K (Liquid for mNGS)

Proteinase K (Liquid for mNGS) is a highly active recombinant serine protease derived from Tritirachium album. As a member of the subtilisin family, it possesses a catalytic triad (Asp39-His69-Ser224) and exhibits broad substrate specificity, efficiently degrading a wide range of proteins under both native and denaturing conditions.

Specifically developed for metagenomic next-generation sequencing (mNGS) workflows, this product is manufactured using a specialized purification process to minimize residual nucleic acid contamination while maintaining excellent enzymatic activity. Compared with conventional Proteinase K products, it provides significantly lower background nucleic acid levels, helping improve the sensitivity, accuracy, and reliability of downstream pathogen detection and sequencing analysis.

The enzyme demonstrates excellent tolerance to chaotropic salts commonly used in nucleic acid extraction systems and is free from detectable DNase, RNase, and Nickase contamination. Its ready-to-use liquid format is suitable for both manual and automated sample preparation workflows.

Key Features

Designed for mNGS Applications

Specially developed for metagenomic next-generation sequencing workflows with ultra-low nucleic acid background to support highly sensitive pathogen detection.

Ultra-Low Nucleic Acid Contamination

Minimized residual host nucleic acids help reduce background interference and improve sequencing data quality.

High Enzyme Activity

Produced through directed evolution technology, delivering efficient protein digestion and robust nucleic acid release.

Excellent Guanidine Salt Tolerance

Maintains high activity in extraction systems containing guanidinium isothiocyanate and guanidine hydrochloride.

DNase-, RNase-, and Nickase-Free

No detectable nuclease contamination, ensuring protection of DNA and RNA during extraction and purification.

Ready-to-Use Liquid Format

Optimized for automated extraction platforms and high-throughput molecular testing laboratories.

Applications

Metagenomic Next-Generation Sequencing (mNGS)

Reduces background contamination during sample preparation and supports highly sensitive microbial and pathogen detection.

DNA and RNA Extraction Kits

Facilitates efficient protein digestion and improves nucleic acid recovery from complex biological samples.

Molecular Diagnostic Reagents

Suitable for PCR, qPCR, RT-PCR, and sequencing-based diagnostic workflows.

Genetic Diagnostic Kits

Used for sample pretreatment and nucleic acid purification in clinical and research testing applications.

Chromosomal DNA Preparation

Applicable for preparation of high-molecular-weight genomic DNA, including pulsed-field gel electrophoresis (PFGE) workflows.

Western Blot and Protein Analysis

Suitable for protein digestion and sample preparation in molecular biology research.

In Vitro Diagnostic Reagents

Can be used in enzymatic glycated albumin assays and other IVD reagent development applications.

Performance Data

3-Year Room Temperature Stability

Excellent Guanidine Salt Tolerance

DNase-, RNase-, and Nickase-Free

FAQ

What are the main features of Proteinase K (Liquid for mNGS)?

It is free of DNase contamination and designed for stable enzyme performance in molecular applications. It is part of Hzymes Enzymes’ Proteinase K product line, which includes different versions for specific applications such as NGS and mNGS workflows.

How stable is Proteinase K in liquid form?

According to Hzymes Enzymes product information, Proteinase K can be stored at 25°C for up to 3 years, depending on the formulation and product version.

What application type is the mNGS version designed for?

The mNGS version is designed as part of the Proteinase K product categories used in nucleic acid sample processing workflows, including advanced sequencing-related applications.