mRNA antigen - Catalyzing life Smart Manufacturing for the Future-Hzymes Biotechnology

mRNA antigen

mRNA synthesis

Service introduction

mRNA has the potential to encode almost all proteins. mRNA vaccine technology can be used in antigen preparation to solve the difficulties in protein preparation: such as the long preparation cycle of traditional protein antigens, complex production process, and poor flexibility in production customization. As a platform technology, mRNA has the advantages of simple production process, short production cycle, and high flexibility. It can provide customers with antigen preparation faster and more cost-effectively, and conduct rapid target feasibility verification.

Service highlights

• mRNA has the potential to encode almost all proteins
• mRNA antigens have obvious advantages for difficult-to-prepare antigens such as transmembrane proteins, difficult-to-purify proteins, and poorly stable antigens
• mRNA antigen production process is simple
• mRNA antigen optimization is highly flexible
• mRNA antigen preparation cost is low

Service scope

• Preparation of mRNA antigens for immunization
• Preparation of mRNA antigens for target validation

Case sharing

mRNA antigen application case 1
mRNA immunization with multiple transmembrane antigens - Antigen A

Sequence design
Plasmid preparation
In vitro transcription
Translation level detection
mRNA-LNP preparation
Animal immunization

Sequence design

Antigen A was codon optimized using multiple sequence design software.
Plasmid preparation

Optimize and improve the supercoil ratio of plasmid
In vitro transcription

Obtain high-purity mRNA stock solution
Translation level detection

Translation level detection

mRNA-LNP preparation

Sample	Particle size: nm	PDI	Encapsulation efficiency
Antigen A mRNA-LNP	104.9	0.08	94.93%

Animal immunization

Antigen A mRNA-LNP mouse immune serum titer results

mRNA antigen application case 2
mRNA immunization with multiple transmembrane antigens - Antigen B

Sequence design
Plasmid preparation
In vitro transcription
Translation level detection
mRNA-LNP preparation
Animal immunization

Sequence design

Antigen B was codon optimized using multiple sequence design software.
Plasmid preparation

Optimize and improve the supercoil ratio of plasmid
In vitro transcription

Obtain high-purity mRNA stock solution
Translation level detection

Translation level detection

mRNA-LNP preparation

Sample	Particle size: nm	PDI	Encapsulation efficiency
Antigen B mRNA-LNP	80.89	0.04	96.59%

Animal immunization

Antigen B mRNA-LNP mouse immune serum titer results

Antigen B mRNA-LNP alpaca immune serum titer results

Service process