Lipoprotein Lipase

Product Overview

The determination of triglycerides (TG) in serum can be performed using either chemical or enzymatic methods; currently, the enzymatic method is the predominant approach in clinical practice. Triglycerides are quantitatively measured through an enzymatic cascade initiated by lipoprotein lipase (LPL). LPL catalyzes the hydrolysis of triglycerides into glycerol and free fatty acids, a step that serves as the rate-limiting reaction in this assay system. Subsequently, the resulting glycerol undergoes further conversion via enzymatic reactions involving glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO), generating hydrogen peroxide (H₂O₂). Under the action of peroxidase (POD), H₂O₂ is transformed into a detectable signal. Lipoprotein lipase (LPL) is the first rate-limiting enzyme in TG assays, specifically hydrolyzing natural C8-C18 triglycerides in serum to yield glycerol and free fatty acids, making it well suited for mainstream fully automated biochemical lipid-profile testing platforms worldwide.

Produced through strain engineering, high-density fermentation, and rigorous purification, this product exhibits a dry-powder specific activity of ≥18 U/mg, stable activity across a pH range of 5.0-8.0, excellent thermal stability up to 50°C, and robust tolerance to various surfactants and heavy metal ions. Its reagent compatibility far exceeds that of commonly available enzymes. Contamination by extraneous enzymes and exogenous proteins is strictly controlled, ensuring exceptionally low background levels. When paired with GK (HH1207), G3PO (HH1103-R2), and POD, it forms a complete enzymatic system for TG measurement, ideal for routine clinical serum lipid analysis and mass production of POCT lipid test strips. Moreover, it can support diverse industrial applications, including food-oil processing, degradation of oil-containing wastewater, and hydrolysis of oleaginous compounds in daily-use chemical products, while demonstrating outstanding long-term storage stability.

Key Features

Excellent thermal stability

Which contributes to the stability of TG reagents.

Broader pH stability range

Facilitating the development and formulation of TG reagents.

Resistant to a variety of chemicals

Including surfactants and metal ions.

Chemical Substances	Vendor A	Vendor B	Vendor C	Hzymes
2 mM CaCl₂	99.6%	90.2%	102.3%	104.0%
2 mM FeCl₃	82.3%	82.8%	86.8%	91.7%
2 mM CoCl₂	93.0%	82.2%	83.6%	96.7%
2 mM MnCl₂	92.0%	98.2%	109.6%	92.7%
2 mM ZnCl₂	80.3%	85.3%	88.2%	85.1%
2 mM NiSO₂	90.2%	92.4%	90.8%	90.2%
1 mM CuSO₄	65.1%	61.4%	23.2%	93.0%
2 mM AgNO₃	68.6%	49.3%	19.9%	90.2%
20 mM NaF	93.0%	98.9%	91.9%	97.5%
20 mM NaN₃	94.1%	94.2%	97.2%	95.6%
9 mM EDTA	83.1%	75.0%	16.6%	33.6%
20 mM Borate	82.9%	94.7%	99.8%	87.7%
2 mM NEM	92.4%	97.3%	95.8%	91.5%
1% Triton X-100	104.6%	104.6%	90.0%	94.2%
1% Brij 35	107.6%	100.5%	98.3%	93.4%
0.1% Tween 20	109.3%	90.6%	101.8%	97.5%
0.1% Span 80	107.3%	99.9%	97.0%	109.6%
1% Na-cholate	106.6%	91.5%	90.2%	83.7%
0.1% SDS	83.1%	9.5%	30.5%	59.7%
0.1 M K pH 7.0, 0.4% BSA	101.7%	91.4%	106.6%	90.4%
0.1 M PB-K pH 7.0, 0.4% BSA, 2% CHAPS	101.4%	48.4%	72.4%	79.3%
0.1 M PB-K pH 7.0, 0.4% BSA, 2% Emulgen B-66	104.9%	86.9%	97.1%	94.7%
0.1 M PB-K pH 7.0, 0.4% BSA, 2% PEG 2000	90.6%	88.4%	89.0%	93.6%

Broad substrate specificity and selectivity

It can hydrolyze triglycerides with C8-C18 carbon chains and is compatible with the natural mixed glycerides present in human serum, making it the preferred enzymatic tool for TG assays.

No.	Oil ester substrates	Carbon Num in the fatty acid chain	Vendor 2	Hzymes
1	Olive oil	C18	100%	100%
2	Glycerol tripropionate	C3	0%	0%
3	Glycerol triacetate	C2	0%	0%
4	Glycerol tributyrate	C4	0%	0%
5	Glycerol trihexanoate	C6	5%	0%
6	Glycerol trioctanoate	C8	39%	37%
7	Glyceryl tridecanoate	C10	13%	12%
8	Glyceryl laurate	C12	28%	32%
9	Glyceryl trimyristate	C14	0%	0%
10	Glyceryl tripalmitate	C16	0%	0%
11	Glyceryl trioleate	C18	12%	9%

Applications

Biochemical assay of serum/plasma triglycerides (TG) (mainstream application):

Used in conjunction with glycerol kinase (catalog no. HH1208) and glycerol-3-phosphate oxidase (catalog no. HH1103-R2) for the development and large-scale preparation of TG reagents.

Food industry:

Hydrolysis of milk fat and animal and vegetable oils; flavor enhancement; advanced oil processing.

Household detergents and chemical industry:

Production of detergents, surfactants, cleaning agents, fabric softeners, and basic oil-based raw materials for cosmetics.

Bioenergy:

Degradation of oil-containing wastewater; production of biodiesel feedstock.

Environmental protection:

Treatment of oil-containing wastewater from catering, food processing, and petrochemical industries; bioremediation of oil contamination in water bodies and soils; degradation of lipids and reduction of COD.

Reaction Principle

One unit of enzyme activity is defined as the amount of enzyme required to catalyze the production of 1 μmol of glycerol per minute under conditions of pH 6.5 and 37°C.

Specification

Parameter	Specification
CAS	9029-22-5
EC Number	EC 3.1.1.34
Appearance	Light brown amorphous lyophilized powder
Dry powder specific activity	≥18 U/mg
Protectants	BSA
Optimal pH	7.0-9.0
pH stability	5.0-8.0
Optimal temperature	45-55°C
Temperature stability	Below 50°C (pH 7.5, 30 min)
Dry Powder Accelerated Stability	After 7 days of accelerated testing at 37°C, the residual activity of the dry powder is ≥90%.
Long-term stability	Store at -25 to -15°C for 2 years, maintaining over 90% activity.
MW	30 kDa (SDS-PAGE)
pI	5.9

Available Formats

• HH1008-01 2kU

• HH1008-02 10kU

• HH1008-03 100kU

• HH1008-04 1000kU

• HH1008-99 Customizable

Performance Data

Wide Linear Range & High Reproducibility

The TG reagent exhibits a wide linear range and excellent batch-to-batch reproducibility.

Strong Method Agreement & Extended Stability (37°C: 21 Days; Open-Vial: 30 Days)

The TG reagent exhibits minimal deviation in measured values compared to reagents from well-known manufacturers, and demonstrates excellent stability: its accelerated stability at 37°C can last up to 21 days, while its open-vial stability on the instrument reaches one month.

Parameter	Specification
Methodology	Enzymatic GPO-POD method
Sample Type	Serum / Plasma
Reagent	Single Reagent
Assay	1-point
Reagent blank Abs	≤0.1000
Linearity Range	0.10-11.4 mmol/L. R² ≥0.990
Correlation with Reference reagents	R² = 0.999
Intra-assay Precision	CV ≤ 3%
Inter-assay Precision	Bias ≤ 5%
On-board Stability	30 days at 2-8°C
Simulated accelerated stability	21 days at 37°C

Low Lot-to-Lot Variability & Stable Performance

TG reagents exhibit minimal lot-to-lot variability and stable performance.

Batch No.	Blank Absorbance	Sensitivity (ΔAbs/ΔConc)
Batch 1	249.0	0.1494
Batch 2	245.5	0.1477
Batch 3	245.5	0.1494
CV	0.80%	0.60%