Sarcosine Oxidase

Product Overview

Serum creatinine measurement is one of the routine clinical tests for renal function. As an important biochemical marker, it is used to diagnose and assess renal insufficiency, nephritis, and the efficacy of treatment for uremia caused by severe glomerular lesions, among other kidney diseases. Due to its poor stability and susceptibility to contamination, the alkaline picric acid method has been gradually phased out by major clinical laboratories; meanwhile, the creatine oxidase method has become the primary approach for clinical creatinine assay. Creatine oxidase (EC 1.5.3.1) catalyzes the oxidation of creatine to produce glycine, formaldehyde, and hydrogen peroxide, serving as the rate-limiting enzyme in the Trinder reaction coupled with the enzymatic creatinine assay. It exhibits resistance to interference from formaldehyde byproducts and effectively prevents absorbance drift. This product is compatible with standardized enzymatic creatinine colorimetric systems, featuring an enzyme specific activity of ≥15 U/mg, a protein purity of ≥90%, and tightly controlled levels of contaminating enzymes. It is optimized for the creatinine-specific pH range of 7.0-9.5 and demonstrates excellent long-term stability at 50°C. Compared with commonly available oxidase reagents on the market, this product offers a lower baseline signal and superior assay reproducibility, making it suitable for routine renal function testing in hospital central laboratories, as a reagent for fully automated biochemical analyzers, and for large-scale production of creatinine test strips. Its performance rivals that of leading international imported enzyme raw materials.

Key Features

High specific activity, excellent pH and thermal stability

Enhancing both long-term reagent stability and open-bottle stability.

Low Km value

Improving the sensitivity of creatinine assays for low-concentration samples.

pH Stability

Thermal Stability

Comparable to R Vendor, AK Vendor, T Vendor and other Vendors in terms of Specific activity, thermal stability and Km.

SOX	Hzymes	R Vendor	T Vendor	AK Vendor	K Vendor	A Vendor	D Vendor
Specific activity (U/mg)	36	28	17	32	36.3	27	33
Optimal temperature (°C)	55	55	55	55	45	50	55
Optimal pH	7.5-8.0	7.5-8.0	7.5-8.0	7.5-8.0	7.75	7.5	8.5-9.0
pH stability	6.0-10.0 (25°C, 24 h)	6.0-10.0 (25°C, 24 h)	6.0-10.0 (25°C, 24 h)	6.0-10.0 (25°C, 24 h)	6.5-10.0 (25°C, 24 h)	6.5-10.0 (25°C, 24 h)	6.5-10.0 (25°C, 24 h)
Km Value	5.7 × 10^-3 M	7 × 10^-3 M	7 × 10^-3 M	14 × 10^-3 M	14 × 10^-3 M	7.48 × 10^-3 M	6.87 × 10^-3 M
Half-life at 50°C (min)	139	75	318	Rapid inactivation	88	106	129
NaN₃ (0.1%) incubate at 37°C for 3 h	More than 70% residual	Activation residual less than 50%	Activation residual less than 50%	Activation residual less than 50%	Activation residual less than 50%	Activation residual less than 50%	Activation residual less than 50%

Applications

Core applications: Hydrolyzed creatine, used in conjunction with CAH (catalog no. HH0106), CR (catalog no. HH0203), and CAT (catalog no. HH2901) for the development and large-scale preparation of enzymatic creatinine assays.

Reaction Principle

The quinoneimine dye formed in the reaction can be detected at 555 nm. One unit of enzyme activity is defined as the amount of enzyme required to catalyze the production of 1 μmol of H₂O₂ per minute under conditions of pH 8.0 and 37°C.

Specification

CAS	9029-22-5
EC Number	EC 1.5.3.1
Appearance	Yellow amorphous lyophilized powder
Dry powder specific activity	≥15 U/mg
Protein Purity	≥90% (SDS-PAGE)
Contaminants	Catalase ≤0.03%; Creatininase amidohydrolase ≤0.01%; Creatine amidinohydrolase ≤0.01%; ATPase ≤0.005%
Optimal pH	8.0
pH stability	pH 7.0-9.5 (25°C, 16 h)
Optimal temperature	50°C
Temperature stability	Below 50°C (pH 7.5, 30 min)
Long-term stability	Store at -25 to -15°C for 12 months, maintaining over 90% activity.
Km	5.6 × 10^-3 M (Sarcosine)
MW	45 kDa (SDS-PAGE)
pI	5.3
Inhibitors	SDS, Zn²⁺, Cd²⁺

Available Formats

• HH0301-01 3kU

• HH0301-02 100kU

• HH0301-03 500kU

• HH0301-04 1000kU

• HH0301-99 Customizable

Performance Data

30-Day Open-Vial Stability (On Instrument)

The creatinine reagent exhibits in-instrument open-vial stability for up to 30 days.

Wide Linear Range (Serum: 8,840 μmol/L; Urine: 24,000 μmol/L)

Wide linear range: the maximum concentration of serum parameters can reach 8840 μmol/L, and that of urine parameters can reach 24000 μmol/L.

Robust Stability (37°C: 21 Days; Freeze-Thaw: 3 Cycles)

At 37°C, the reagent remains stable after 7, 14, and 21 days of accelerated storage; at -20°C, it retains its stability after three freeze-thaw cycles.

Strong Anti-Interference Performance

Unique anti-interference technology, strong anti-interference ability.

Interferents	Vitamin C	Bilirubin	TG	Hemoglobin	Calcium hydroxybenzene sulfonate
Vendor A	≤40 mg/dL	≤20 mg/dL	≤1000 mg/dL	≤200 mg/dL	≤0 mg/dL
Hzymes	≤50 mg/dL	≤40 mg/dL	≤1000 mg/dL	≤500 mg/dL	≤64 mg/dL