Specification
| Appearance | White amorphous powder, lyophilized |
| Purity(SDS-PAGE) | ≥90% |
| Enzyme powder specific activity | ≥420.0 U/mg |
| NADH/NADPH oxidase | ≤0.1% |
| Hexokinase | ≤0.001% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
Properties
| EC number | 1.1.1.49 (Recombinantfrom microorganism) | |
| Molecular weight | 57 kDa (SDS-PAGE) | |
| Isoelectric point | 4.6 | |
| Michaelis constants | 3.2×10-4M(NAD+),2.6×10-4M(G-6-P) | |
| Inhibitors | SDS,Zn2+,Fe3+ | |
| Optimum pH | 8 | Fig.1 |
| Optimum temperature | 45℃ | Fig.2 |
| pH stability | pH 5.5-10.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 40℃ (pH 7.5,30 min) | Fig.4 |
| Storage stability | At least one year at -25～-15℃ | Fig.5 |
| Stabilizers | BSA | |
Applications
This enzyme is useful for enzymatic determination of glucose or ATP when coupled with hexokinase.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADH per min under the conditions described below.
