Glucose-6-Phospate Dehydrogenase

Product Overview

Cardia-cspecific creatine kinase-MB isoenzyme (CK-MB) is a heterodimer composed of M and B subunits and represents the most specific enzyme in the cardiac enzyme panel. An elevation of serum CK-MB activity is widely recognized as a key diagnostic marker for acute myocardial infarction and for confirming the presence of myocardial necrosis, and it is regarded as the "Gold Standard" in cardiac enzymology. This biomarker can be used for the early diagnosis of acute myocardial infarction (AMI). Glucose-6-phosphate dehydrogenase (G6PDH) serves as a critical raw material for assays measuring glucose (GLU), creatine kinase (CK), creatine kinase MB isoenzyme (CKMB), carbon dioxide, and other analytes; it also functions as an internal control enzyme reference for HCY and liver function assay kits. This product is a recombinant G6PDH, produced via high-density microbial fermentation followed by column chromatographic purification, manufactured on an industrial scale with consistently stable supply. It remains stable across a pH range of 5.0-9.5 and exhibits excellent compatibility with various assay systems. Its outstanding thermal stability makes it well suited for long-term storage of liquid reagents. With a protein purity ≥90% and rigorously controlled batch-to-batch impurity levels, HH0601 is compatible with CK reagents, CKMB reagents, glucose (HK method) reagents, and CO₂reagents, delivering superior assay performance. Hzymes independently develops and manufactures core raw materials such as G6PDH (HH0601), HK (HH0705), PEPC (HH1902), MDH (HH1802), and CKMM antibodies (HH0805). With full-process quality control, we offer customized services for both raw materials and reagents, helping IVD manufacturers worldwide reduce costs, improve efficiency, and achieve mutual success.

Key Features

High specific enzyme activity (＞900U/mg) and high specific activity

of the lyophilized powder (≥420U/mg), allowing for lower loading amounts in assays and minimizing interference with the assay system.

Enzyme activity remains stable across a broad pH range of 5.0-9.5,

matching that of competing products and exhibiting excellent compatibility with assay systems.

Thermal stability is comparable to that of leading competitors.

G6PDH	Hzymes	Vendor R	Vendor AK	Vendor T	Vendor A	Vendor LM
Specific activity(U/mg)	964	149	90	1130	1000	900
Optimal temperature	50°C	60°C	50°C	50°C	50°C	50°C
Optimal pH	8.0	8.0	8.0	8.0	8.0	8.0
pH stability	5.0-9.5	5.0-9.5	5.0-9.5	5.0-9.5	5.0-9.5	5.0-9.5
Half-life at 42°C(min)	>250	>250	>250	>250	>250	>250

Applications

Cardiac enzyme assays:

G6PDH compatible hexokinase (HH0705) and CKMM antibody (HH0805) are used for the development and largescale preparation of creatine kinase (CK) and creatine kinase isoenzyme CK-MB reagents (immunoinhibition method).

Blood glucose assays:

G6PDH compatible hexokinase (HH0705) is employed in the development and largescale formulation of glucose (GLU) assay reagents.

Carbon dioxide assays:

G6PDH, together with glucose, ATP, HK (HH0705), PEPC (HH1902), and MDH (HH1802), is used to construct an NADH regeneration system for the development and largescale preparation of CO₂assay reagents.

Reaction Principle

The amount of NADH produced in the reaction can be measured using a spectrophotometer at a wavelength of 340nm. One unit of enzyme activity is defined as the amount of enzyme required to generate 1μmol of NADH per minute under reaction conditions of pH7.8 and 37°C.

Specification

CAS	9001-40-5
EC Number	EC 1.1.1.49
Appearance	White amorphous lyophilized powder
Dry powder specific activity	≥420.0U/mg
Protein Purity	≥90%(SDS-PAGE)
Protectants	BSA
Contaminants	NADH/NADPH oxidase≤0.1%; Hexokinase≤0.001%; Glutathione reductase≤0.001%;Phosphoglucoseisomerase≤0.001%
Optimal pH	pH8.0
pH stability	pH5.5-10.0 (25°C, 16 h)
Optimal temperature	45°C
Km	3.2×10^-4M (NAD+), 2.6×10^-4M (Glucose-6-phosphate)
Temperature stability	Below 40°C (pH7.5, 30 min)
Long-term stability	Stored at -20°C for 12 months, maintaining over 90% activity.
MW	57kDa(SDS-PAGE)
pI	4.6
Inhibitors	SDS, Zn²⁺, Fe³⁺

Available Formats

• HH0601-01 10kU

• HH0601-02 100kU

• HH0601-03 500kU

• HH0601-04 100kU

• HH0601-99 Customizable

Performance Data

Hzymes' CK-MB reagent exhibits a wide linear range of 4-600 U/L

R² > 0.99 Correlation with Leading Commercial Reagents

The CK-MB reagent from Hzymes demonstrates excellent correlation with results obtained using reagents from leading manufacturers for random samples (R² > 0.99), with an average bias of less than ±10%.

28-Day Onboard Stability After Opening

The CK-MB reagent exhibits excellent stability, remaining stable as new for 28 days after opening when stored on the instrument.

R² > 0.99 Correlation with Leading Commercial Reagents

The glucose assay reagent (HK method) demonstrates excellent correlation with results obtained using reagents from leading manufacturers (R² > 0.99), with a relative bias of less than ±10%.

Proven Stability After 7-Day Accelerated Aging at 37°C

After 7 days of accelerated aging at 37°C, the glucose assay reagent (HK method) demonstrated good correlation with the results obtained using refrigerated reagents for clinical samples, with a bias of less than ±10%.