Product Overview

SUMO proteases recognize the tertiary structure of the ubiquitin-like SUMO domain and cleave the peptide bond following the Gly-Gly bond in the x-Gly-Gly-x sequence at the C-terminus of the SUMO domain

Key Features

High Cleavage Specificity

Recognizes the native tertiary structure of the SUMO domain and specifically cleaves the peptide bond immediately following the C-terminal Gly-Gly motif, minimizing off-target proteolysis.

Efficient Enzymatic Activity

Provides reliable cleavage performance under recommended reaction conditions, supporting efficient processing of SUMO fusion proteins.

Precise Native Sequence Recovery

Removes SUMO tags without introducing additional amino acid residues, enabling the generation of target proteins with their native N-termini.

Recombinant Protein Processing Compatible

Suitable for a variety of recombinant protein expression and purification workflows involving SUMO fusion constructs.

Applications

SUMO Fusion Tag Removal

Widely used for the specific cleavage of recombinant SUMO fusion proteins during downstream purification and processing.

Recombinant Protein Production

Applicable to the preparation of recombinant proteins requiring native N-terminal sequences after tag removal.

SUMOylation Studies

Can be used in studies involving naturally SUMO-modified proteins and the investigation of SUMO-related biological processes.

Process Development and Optimization

Supports process development, purification optimization, and scale-up activities in protein production workflows utilizing SUMO tags.