Product Description
mRNA Cap 2´ -O-methyltransferase was derived from a recombinant E. coli strain that carries the gene for the vaccinia mRNA Cap 2´-O-Methyltransferase. This enzyme adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
Product Information
| Product Name | Cat. No. | Size |
| mRNA Cap2'-O-Methyltransferase | HBP000701 | 2.5KU/0.05mL |
| HBP000702 | 10KU/0.2mL | |
| HBP000703 | 500KU/10mL | |
| HBP000704 | 5000KU/100mL | |
| HBP000705 | 50000KU/1000mL | |
| HBP000706 | 50KU/1mL |
Shipping and Storage
Stored at -25 °C to -15°C （ Avoid repeated freeze-thaw cycles）
Unit Definition
One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt capped RNA transcript in 1 hour at 37°C.
