Product Overview

mRNA Cap 2'-O-Methyltransferase is a recombinant enzyme produced by expression in Escherichia coli (E. coli). It catalyzes the transfer of a methyl group from the methyl donor S-Adenosylmethionine (SAM) to the ribose 2'-hydroxyl group of the first nucleotide of the Cap 0 structure, generating the Cap 1 structure of mRNA.

Cap 1 modification plays a critical role in improving mRNA performance by enhancing translation efficiency, increasing protein expression levels, and reducing innate immune recognition after mRNA delivery. This enzyme is designed for high-efficiency enzymatic mRNA capping workflows and is suitable for mRNA vaccine development, gene therapy research, and large-scale GMP manufacturing.

Manufactured under a GMP-compliant production environment, this product features animal origin-free processing, strict impurity control, and consistent batch-to-batch performance to support applications from preclinical research to commercial mRNA drug production.

Key Features

GMP-Grade Manufacturing

• Produced under GMP-compliant conditions with animal origin-free processing.

• Free of β-lactam antibiotics.

• Supported by comprehensive quality control standards and DMF filing.

• Ensures high batch-to-batch consistency for research and commercial manufacturing.

High Capping Efficiency

• Efficiently converts Cap 0 mRNA into Cap 1 mRNA.

• Achieves >95% capping efficiency for standard mRNA sequences.

• Supports the production of highly active mRNA products with improved translation performance.

Low Residual Impurities

• Nuclease-free formulation to protect mRNA integrity.

• Low residual levels of host cell DNA (HCD), host cell protein (HCP), microorganisms, metal ions, and endotoxin.

• Meets stringent quality requirements for mRNA drug development and manufacturing.

Pre-Denaturation-Free Capping Compatibility

• Enables efficient capping without mandatory pre-denaturation of mRNA templates.

• Simplifies workflow operation and reduces process complexity.

• Facilitates scalable mRNA production processes.

Patent-Friendly Solution

• Designed without patent restrictions, providing greater flexibility for mRNA manufacturing process development.

• mRNA enzymatic capping

• Enables efficient conversion of Cap 0 mRNA to Cap 1 mRNA for enhanced biological performance.

• mRNA vaccine development

• Supports the production of high-quality capped mRNA candidates with improved translation efficiency and reduced immunogenicity.

• Gene therapy research

• Suitable for mRNA-based therapeutic development requiring optimized mRNA stability and expression.

• Preclinical and GMP manufacturing

• Applicable for early-stage research, process development, and large-scale commercial mRNA production.

Performance Data

High-Efficiency Cap 1 Formation

The mRNA Cap 2'-O-Methyltransferase demonstrates excellent catalytic activity and delivers high capping efficiency across different mRNA templates.

• Capping efficiency exceeds 95% for standard mRNA sequences.

• Provides consistent Cap 1 modification performance for diverse mRNA applications.

Simplified Capping Workflow Performance

The enzyme supports efficient capping reactions with or without 65°C pre-denaturation treatment.

• Comparable capping efficiency achieved under both workflows.

• Eliminates the requirement for additional denaturation steps.

• Enables a simpler and more scalable mRNA production process.

Superior mRNA Protection and Translation Potential

Cap 1 modification improves mRNA recognition by cellular translation machinery while reducing activation of innate immune pathways, contributing to enhanced protein expression and improved mRNA therapeutic performance.

FAQ

Q1: What is mRNA Cap 2'-O-Methyltransferase used for?

A: mRNA Cap 2'-O-Methyltransferase is used for enzymatic conversion of Cap 0 mRNA into Cap 1 mRNA by transferring a methyl group from SAM. Cap 1 modification improves translation efficiency and reduces innate immune response.

Q2: What is the capping efficiency of this enzyme?

A: The enzyme achieves a capping efficiency of over 95% for standard mRNA sequences.

Q3: Does the capping process require mRNA pre-denaturation?

A: No. This enzyme supports a pre-denaturation-free capping workflow, simplifying operation and improving compatibility with large-scale manufacturing processes.

Q4: What are the storage and transportation conditions?

A: The product should be transported below 0°C and stored at -25°C to -15°C.

Q5: Is this product suitable for GMP manufacturing?

A: Yes. The product is manufactured under GMP-compliant conditions with animal origin-free processing, strict impurity control, and quality documentation support for GMP mRNA production.

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