Product Overview

LAMP Fluorescent Master Mix (In Situ Lyophilization) is a ready-to-use reagent designed for high-performance loop-mediated isothermal amplification (LAMP) assays. The product contains a complete reaction system including reaction buffer, Bst DNA polymerase enzyme mix, lyophilization protectants, and fluorescent dye components. The buffer system is pre-optimized with Mg²⁺ and dNTPs required for efficient DNA amplification.

This master mix is supplied in a lyophilized (freeze-dried) format, enabling enhanced stability and convenient room-to-assay workflow. Users only need to add primers and template DNA to initiate the reaction, significantly simplifying assay setup and reducing preparation time. The product is suitable for DNA isothermal amplification applications in research and diagnostic workflows.

Product components

Key Features

Ready-to-use lyophilized format:

Pre-assembled reaction system minimizes pipetting steps and reduces contamination risk.

All-in-one LAMP system:

Includes Bst DNA polymerase, optimized buffer, Mg²⁺, dNTPs, stabilizers, and fluorescent dye components.

In situ lyophilization technology:

Enhances reagent stability and supports long-term storage and transport at 2–8°C.

Simple workflow:

Only primers and template are required for reaction setup, improving experimental efficiency.

High amplification efficiency:

Designed for robust DNA isothermal amplification with strong sensitivity and specificity.

Flexible packaging options:

Available in multiple strip formats (8 T/strip × 12 strips / 120 strips / 1200 strips) for different throughput needs.

Applications

• DNA isothermal amplification (LAMP-based detection)

• Molecular diagnostics research

• Pathogen nucleic acid detection

• Point-of-care testing (POCT) assay development

• Rapid DNA screening workflows

• Fluorescence-based real-time LAMP detection systems

FAQ

What is “in situ lyophilization”?

In situ lyophilization refers to freeze-drying the complete reaction system directly in the final container, forming a ready-to-use solid-state LAMP reagent.

What components are included in this product?

It includes reaction buffer, Bst DNA polymerase enzyme mix, lyophilization protectants, and fluorescent dye components. The buffer contains Mg²⁺, dNTPs, and other amplification cofactors.

What is the main advantage compared with liquid LAMP systems?

It significantly simplifies assay setup, reduces contamination risk, and improves reagent stability and transport convenience.

What do users need to add for amplification?

Only primers and template DNA are required to start the reaction.

What storage conditions are required?

The product is transported and stored at 2–8°C with a validated shelf life of 12 months.

What applications is it suitable for?

It is suitable for DNA isothermal amplification, POCT development, molecular diagnostics, and fluorescence-based LAMP detection systems.