Product Overview

Proteinase K Storage Buffer is a dedicated supporting diluent specifically optimized for lyophilized Proteinase K (Protease K) formulations. It is designed to maintain enzyme stability, preserve catalytic activity, and ensure consistent performance during storage and reconstitution.

The buffer is composed of Glycerol, Sodium Acetate, and Acetic Acid, providing an optimized chemical environment that supports long-term enzyme stability while minimizing activity loss during transportation and storage.

This formulation is suitable for molecular biology and molecular diagnostic workflows where consistent Proteinase K performance is required for reliable nucleic acid extraction and sample preparation.

Key Features

Optimized Enzyme Stability System

Formulated specifically for lyophilized Proteinase K to maintain enzyme integrity and functional activity after reconstitution.

No Contaminating Enzymes

Manufactured under strict quality control to ensure absence of DNase, RNase, and other contaminating enzymatic activities.

High Enzyme Activity Support

Direct enzyme evolution–supported Proteinase K ensures higher specific activity when used with the storage buffer system.

High Salt Tolerance Compatibility

Stable performance in the presence of chaotropic salts such as guanidine hydrochloride and guanidine isothiocyanate, supporting nucleic acid extraction workflows.

Residue-Free Purity

Free from DNase, RNase, and nickase contamination, ensuring compatibility with sensitive downstream molecular applications.

mNGS-Grade Cleanliness (No Background Contamination)

Microbial and bioburden-controlled manufacturing process suitable for high-sensitivity molecular diagnostic applications.

Applications

DNA and RNA Extraction

Efficient protein and nuclease degradation during nucleic acid purification, improving DNA and RNA yield and purity.

Molecular Diagnostic Reagents

Widely used in PCR, qPCR, RT-PCR, and other molecular diagnostic workflows requiring high-quality nucleic acid preparation.

Genetic Diagnostic Kits

Applied in sample pretreatment and nucleic acid extraction steps in genetic testing workflows.

Biopharmaceutical Processing

Supports removal of protein impurities during production and purification of nucleic acid-based therapeutics and biological products.

Tissue and Cell Sample Preparation

Facilitates efficient digestion of proteins in biological samples, enabling improved recovery of non-protein analytes.

Performance Data

3-Year Room Temperature Stability

Both Proteinase K lyophilized powder and reconstituted solution demonstrate stable performance when stored at room temperature for up to three years.

Excellent Guanidine Salt Tolerance

Maintains enzymatic activity in the presence of:

• Guanidine isothiocyanate

• Guanidine hydrochloride

Enabling robust performance in chaotropic salt-based lysis systems.

High Purity: DNase / RNase / Nickase Free

Verified absence of:

• DNase activity

• RNase activity

• Nickase contamination

Ensures compatibility with sensitive nucleic acid workflows and downstream enzymatic reactions.

FAQ

What is Proteinase K Storage Buffer used for?

It is a supporting diluent specifically optimized for lyophilized Proteinase K powder.

What are the main components of the storage buffer?

The buffer mainly contains glycerol, sodium acetate, and acetic acid.

What are the storage and transportation conditions?

It is stored at 2–8°C with a shelf life of 24 months, and requires low-temperature transportation.

Does the buffer affect enzyme activity?

It is designed as a dedicated formulation to support the stability of lyophilized Proteinase K without introducing miscellaneous enzyme contamination.