Service Overview

ELISA (Enzyme-Linked Immunosorbent Assay) is a detection method based on antigen-antibody interactions in vitro, combining the specificity of antigen-antibody reactions with the high catalytic efficiency of enzymes. It achieves sensitivity at the pg/mL level and is one of the most commercially mature immunoassay technologies, widely applied in medical research, clinical diagnostics, and biopharmaceuticals. ELISA methods can be categorized into two types based on application:
Quantification of biomolecules: Monitoring antibody expression (stable/transient transfection, fermentation processes), cytokine levels, pharmacokinetics of antibody drugs, biomarker quantification, and immunogenicity testing.
Affinity assessment or screening: Hybridoma titer determination, phage display library screening, and evaluation of antibody affinity for target proteins or cell surface receptors.
Hzymes boasts a professional R&D and technical service team, having independently developed multiple ELISA kits. We provide tailored experimental designs, efficient detection method development, comprehensive validation, and customized ELISA testing services or kit development for biopharmaceutical clients.

Service Scope

Direct ELISA:
Advantages: Simple, ideal for antibody quantification.
Limitations: Requires conjugated detection antibodies, high background, not suitable for multiplexing.
Indirect ELISA:
Advantages: Signal amplification via secondary antibodies, higher sensitivity than direct ELISA.
Limitations: More steps, potential for nonspecific binding.
Sandwich ELISA:
Advantages: High specificity using paired antibodies.
Limitations: Time-consuming and costly antibody pair development.
Competitive ELISA:
Applications: Quantification of low-abundance analytes, immune response evaluation.
We offer services and kit development for direct, indirect, sandwich, and competitive ELISA, including inhibitor screening, antigen-antibody binding assays, bioactivity analysis, pharmacokinetics, and immunogenicity testing.

Service Advantages

• In-house production of critical reagents:

Self-developed antigens, antibodies, and standards.

• Expert antibody labeling:

Extensive experience in HRP and biotin conjugation.

• Superior performance:

High sensitivity, stability, and accuracy.

• Scalable production:

Controlled batch-to-batch consistency for large-scale manufacturing.

• Rapid delivery:

Customized kits delivered within 2 weeks after reagent preparation.

• Technical expertise:

Capabilities in multiplex ELISA and small-molecule (e.g., nucleic acid) detection.

Case sharing

Universal nuclease detection kit

1.0 HBP000111

Technical principle: ELISA double antibody sandwich method;
Detection range: 0.2~20ng/mL;
Detection limit: ≤0.06ng/mL;
Minimum limit of quantification: 0.2ng/mL;
Precision: Intra-batch CV≤10%, inter-batch CV ≤15%;
Accuracy: Recovery rate range 80%~120%;
Specificity: No cross-reaction with irrelevant proteins such as trypsin, RNase A, DNase I;
Adaptability: It can identify universal nucleases of multiple brands, but the standard product used with this reagent is the universal nuclease of Hzymes, so it is recommended to use the corresponding product to establish a standard curve when detecting other brands of universal nucleases to ensure the accuracy of the measurement results;
Operation process: 6 steps, incubation time 2h, wash 10 times;

2.0 HBP000116
Technical principle: ELISA double antibody sandwich method;
Detection range: 0.02~2ng/mL;
Detection limit: ≤0.01ng/mL;
Lowest limit of quantification: 0.02ng/mL;
Precision: Intra-batch CV≤10%, inter-batch CV ≤15%;
Accuracy: Recovery rate range 80%~120%;
Specificity: No cross-reaction with irrelevant proteins such as trypsin, RNase A, DNase I;
Adaptability: It can identify multiple brands of universal nucleases, but the standard product of this reagent is the universal nuclease of Hzymes, so it is recommended to use the corresponding product to establish a standard curve when detecting other brands of universal nucleases to ensure the accuracy of the measurement results;
Operation process: 8 steps, incubation time 2h30min, wash 15 times;

T7 RNAP Residue Detection Kit (ELISA Method)

Project process