Homocysteine (HCY) Assay Kit (Enzymatic Method)

Protease K |Creatinase lamp |dNTP |All-purpose nuclease mRNA

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80ml: HOEM1901-01

1000ml: HOEM1901-02

5L: HOEM1901-03

Cat. No: HOEM1901

Storage and stability:
Up to expiration date indicated on the label, when stored unopened at 2-8℃ and protected from light.
Once opened, the reagents are stable for 28 days when refrigerated on the analyzer or refrigerator. Contamination of the reagents must be avoided. Do not freeze the reagents.

Technical Support

Product Overview

The oxidized Homocysteine is converted into free Homocysteine. The free Homocysteine reacts with serine under the catalysis of CBS to form L-cysteine. L-cysteine is then catalyzed by CBL to generate Homocysteine, pyruvic acid and NH₃. The pyruvic acid produced in this cycle reaction can be detected using lactate dehydrogenase (LDH) and NADH. The rate of NADH converting to NAD is proportional to the Homocysteine content in the sample.

Key Features

• The self-production of the core enzyme: lower reagent costs and minimal batch-to-batch variations.

• Excellent stability: the simulated accelerated stability at 37°C can last up to 14 days.

Applications

Quantitative HCY Testing in Serum/Plasma (Photometric Systems)

In vitro test for the quantitative determination of HCY concentration in serum or plasma on photometric systems.

Cardiovascular Risk Assessment Marker

Homocysteine (Hcy) is a thiol-containing amino acid produced by the intracellular demethylation of methionine. Total homocysteine (tHcy) represents the sum of all forms of Hcy including forms of oxidized, protein bound and free. Elevated level of tHcy has emerged as an important risk factor in the assessment of cardiovascular disease. Excess Hcy in the blood stream may cause injures to arterial vessels due to its irritant nature, and result in inflammation and plaque formation, which may eventually cause blockage of blood flow to the heart.

Performance

Parameter	Specification
Methodology	Enzymatic method
Sample Type	Serum / Plasma
Reagent	Double Reagent
Assay	2-point
Reagent blank Abs	≥0.8
Linearity Range	2-50 μmol/L. R² ≥0.990
Correlation with Reference reagents	R² = 0.995
Intra-assay Precision	CV ≤ 5%
Inter-assay Precision	CV ≤ 10%
On-board Stability	30 days at 2-8°C
Simulated accelerated stability	14 days at 37°C