Specification
| Appearance | White to faint yellow lyophilized powder |
| Purity(SDS-PAGE) | ≥90.0% |
| Enzyme powder specific activity | ≥400.0 U/mg |
| Catalase | ≤0.1% |
| ATPase | ≤0.001% |
| Proteases | ≤0.001% |
| Creatine amidinohydrolase | ≤0.001% |
Properties
| ECnumber | 3.5.2.10(Recombinantfrom microorganism) | |
| Molecular weight | 29kDa (SDS-PAGE) | |
| Isoelectric point | 5.3 | |
| Michaelis constants | 5.0×10-2M(Creatinine), 8.0×10-2M(Creatine) | |
| Inhibitors | Hg2+,Cu2+,Fe3+ | |
| OptimumpH | 7.0-8.0 | Fig.1 |
| Optimum temperature | 65℃ | Fig.2 |
| pH stability | pH 5.5-10.0 (25℃,16h) | Fig.3 |
| Thermal stability | Below 65℃ (pH8.0,30min) | Fig.4 |
| Storage stability | At least one year at-25～-15℃ | Fig.5 |
| Stabilizers | BSA,sugar | |
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidohydrolase and sarcosine oxidase.
Unit definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of creatine per min under the conditions described below
