Glycerol kinase

Product Overview

Glycerol kinase (GK) is the core rate-limiting enzyme for enzymatic triglyceride (TG) detection, catalyzing ATP-dependent glycerol phosphorylation to connect downstream reaction of LPL hydrolysis, which determines low-value detection accuracy of TG reagents. This recombinant modified enzyme has dry powder specific activity ≥40 U/mg and protein purity ≥90%, with ultra-wide pH stability of 4.5-10.0 and excellent thermal stability below 65°C. Optimized substrate Km value reduces endogenous serum glycerol background interference, with strict contaminant control. It achieves perfect compatibility with HH1008 and HH1103-R2, suitable for global biochemical analyzers and dual-formula lipid diagnostic reagent development. It owns low batch-to-batch variation and outstanding on-board open-bottle stability, ideal for reagent mass production and cross-border storage in high-temperature areas.

Key Features

Excellent thermal stability

Which helps enhance the stability of TG reagents.

Ultra-wide stability range of pH 4.5-10.0

Offering greater reagent compatibility and more room for optimization.

Resists a wide range of surfactants and metal ions

Exhibiting excellent reagent compatibility.

Effect of Various Chemicals on Glycerol Kinase (37°C, 24 h Residual Activity)
No.	Chemicals	Concentration	Vendor A	Vendor B	Hzymes HH1207
1	MgCl₂	1 mM	93.6%	94.1%	93.4%
2	CaCl₂	1 mM	93.6%	101.1%	91.0%
3	FeSO₄	1 mM	95.5%	98.1%	77.0%
4	FeCl₃	1 mM	94.5%	92.1%	72.1%
5	CoCl₂	1 mM	95.5%	99.1%	75.4%
6	MnCl₂	1 mM	93.6%	97.1%	90.2%
7	ZnCl₂	1 mM	0.0%	0.0%	1.6%
8	NiSO₄	1 mM	95.5%	97.1%	77.3%
9	CuSO₄	1 mM	95.5%	93.7%	89.4%
10	AgNO₃	1 mM	0.0%	0.0%	1.6%
11	DTT	1 mM	98.2%	101.1%	101.6%
12	NaF	1 mM	93.6%	97.1%	103.8%
13	NaN₃	1 mM	100.0%	96.1%	104.1%
14	EDTA	5 mM	97.3%	93.1%	103.1%
15	Borate	50 mM	95.5%	101.1%	96.7%
16	IAA	1 mM	97.3%	95.1%	100.0%
17	NEM	1 mM	8.7%	0.3%	45.1%
18	Hydroxylamine	1 mM	98.2%	97.1%	96.7%
19	Triton X-100	1%	94.9%	108.0%	104.6%
20	Brij 35	0.10%	97.3%	106.1%	115.6%
21	Tween 20	0.10%	99.5%	100.1%	104.9%
22	Span 20	0.10%	101.0%	102.1%	103.4%
23	Na-cholate	0.50%	96.5%	105.1%	125.4%
24	SDS	0.50%	0.0%	1.5%	0.8%
25	DAC	0.13%	0.5%	0.8%	1.6%
26	ProClin 300	0.25 g/L	N/A	N/A	28.5%
27	Sodium hyodeoxycholate	1.5 g/L	N/A	N/A	97.0%
28	APE-10	1 g/L	N/A	N/A	108.2%
29	APEP-105	1 g/L	N/A	N/A	107.6%
30	APEP-108	1 g/L	N/A	N/A	107.8%
31	Sodium thiosulfate	0.05 g/L	N/A	N/A	88.7%

Applications

Lipid profile testing—triglyceride assay: The core reagents are the accompanying G3PO (HH1103R2) and LPL (HH1008), which are used for the development and large-scale preparation of triglyceride assay kits.

Reaction Principle

One unit of enzyme activity is defined as the amount of enzyme required to catalyze the consumption of 1 μmol of glycerol per minute under conditions of pH 7.9 and 37°C.

Specification

CAS	9030-66-4
EC Number	EC 2.7.1.30
Appearance	White to pale yellow amorphous lyophilized powder
Dry powder specific activity	≥40 U/mg
Protein purity	≥90%
Protectants	BSA
Optimal pH	7.5-8.5
pH stability	4.5-10.0
Optimal temperature	50°C
Temperature stability	Below 65°C (pH 7.5, 30 min)
Long-term stability	Store at -25 to -15°C for 18 months, maintaining over 90% activity.
MW	58 kDa (SDS-PAGE)
Km	3.7 × 10^-6 M (Glycerol) 5.6 × 10^-5 M (ATP)

Available Formats

• HH1207-01

• HH1207-02

• HH1207-03

• HH1207-04

• HH1207-99 Customizable

Performance Data

Wide Linear Range & High Reproducibility

The TG reagent exhibits a wide linear range and excellent batch-to-batch reproducibility.

Strong Method Agreement & Stability (37°C, 21 Days; Open-Vial, 30 Days)

The TG reagent exhibits minimal deviation in measured values compared to reagents from well-known manufacturers, and demonstrates excellent stability: its accelerated stability at 37°C can last up to 21 days, while its open-vial stability on the instrument reaches one month.

Parameter	Specification
Methodology	Enzymatic GPO-POD method
Sample Type	Serum / Plasma
Reagent	Single Reagent
Assay	1-point
Reagent blank Abs	≤0.1000
Linearity Range	0.10-11.4 mmol/L; R² ≥0.990
Correlation with Reference reagents	R² = 0.999
Intra-assay Precision	CV ≤3%
Inter-assay Precision	Bias ≤5%
On-board Stability	30 days at 2-8°C
Simulated accelerated stability	21 days at 37°C

Low Lot-to-Lot Variability

TG reagents exhibit minimal lot-to-lot variability and stable performance.

Batch No.	Blank Absorbance	Sensitivity (ΔAbs/ΔConc)
Batch 1	249.0	0.1494
Batch 2	245.5	0.1477
Batch 3	245.5	0.1494
CV	0.80%	0.60%