Product Overview

The LAMP Colorimetric Master Mix (In Situ Lyophilization) is a ready-to-use reagent system designed for rapid and visual DNA isothermal amplification. The product contains a reaction buffer, Bst DNA polymerase enzyme mix, lyophilized protectants, and chromogenic dye components. The reaction buffer provides essential components including Mg²⁺ and dNTPs required for efficient amplification.

The system is supplied in a lyophilized powder format, enabling enhanced stability and convenient storage. Users only need to add primers and template DNA before use, significantly simplifying workflow and reducing preparation time.

During amplification, results are directly visible by a color change: negative reactions remain red, while positive reactions turn yellow, enabling fast and intuitive result interpretation without the need for specialized instrumentation.

Product components

Key Features

Ready-to-use lyophilized format

Pre-formulated and freeze-dried system for improved stability and ease of transport.

Simple workflow, minimal setup

Only primers and DNA template need to be added before reaction setup.

Visual colorimetric detection

Clear result interpretation:

• Negative: red

• Positive: yellow

Fast isothermal amplification

Based on LAMP (Loop-mediated Isothermal Amplification) technology for rapid DNA detection.

Stable formulation

Includes lyophilized protectants to ensure enzyme and reagent stability during storage and transportation.

Flexible packaging options

Available in multiple strip formats to support different throughput needs:

• HMD5215-01: 8 T/strip × 12 strips

• HMD5215-02: 8 T/strip × 120 strips

• HMD5215-03: 8 T/strip × 1200 strips

Applications

• DNA isothermal amplification assays

• Rapid molecular detection workflows

• Point-of-care testing (POCT) molecular diagnostics

• Pathogen detection in research and applied laboratories

• Field-deployable nucleic acid testing systems

• Visual detection-based screening assays

FAQ

Does Bst polymerase have reverse transcriptase activity?

No. Hzymes Bst enzyme provides strand displacement DNA polymerase activity but does not include reverse transcriptase activity.

What does “in situ lyophilization” mean?

A: It means the reaction system can be directly mixed with lyophilization protectants and freeze-dried in the final reaction vessel, enabling conversion into a ready-to-use lyophilized diagnostic format.

Does the product contain UDG?

A: No. Standard products do not include UDG, but customized versions are available.

What is the detection method ranking in performance?

• Sensitivity: Probe-based ≈ Fluorescent > Colorimetric

• Specificity: Probe-based ≈ Fluorescent > Colorimetric

What are the advantages of Bst V2.0 in this system?

Faster amplification, improved salt tolerance, and enhanced stability. It is not compatible as a Taq polymerase replacement.

How can LAMP sensitivity be improved?

Optimize enzyme dosage, primer concentration, and reaction conditions. Bst V2.0 can improve overall efficiency.

How can false positives or non-specific amplification be reduced?

• Use WS enzyme variants

• Add additives such as betaine

• Optimize primer design and reaction system balance