Preparation and specification
| Appearance | White amorphous powder, lyophilized |
| Protein purity | ≥90% (SDS-PAGE) |
| Activity | ≥20 U/mg solid |
| Catalase |
≤0.03% |
| NADH/NADPH oxidase | ≤0.01% |
Properties
| EC number | 1.7.3.3 (Recombinantfrom microorganism) | |
| Molecular weight | 40 kDa (SDS-PAGE) | |
| Isoelectric point | 5.1 | |
| Michaelis constants | 2.1×10-5M (Urate) | |
| Inhibitors | Fe3+,Co2+,Cu2+,Mn2+,Zn2+ | |
| Optimum pH | 8.5 | Fig.1 |
| Optimum temperature | 37℃ | Fig.2 |
| pHstability | pH 5.5-10 .0 (25 ℃, 16 h) | Fig.3 |
| Thermal stability | 60 ℃ (pH 8.5, 30 min) | Fig.4 |
| Storage stability | At least one year at -25~-15 ℃ | Fig.5 |
| Stabilizer | Borate, EDTA and nonionic surfactant | |
Applications
This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Unit definition
One unit (U) is defined as the amount of enzyme which consumes 1 μmol of uric acid per min under the conditions described below.
