Plasmid Preparation Service - Catalyzing life Smart Manufacturing for the Future-Hzymes Biotechnology

Plasmid Preparation Service

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Service Overview

Hzymes Biotech provides plasmid preparation services for mRNA and cell gene therapy clients. Our proprietary plasmid backbone ensures the stability of the mRNA plasmid’s Poly(A) tail. We offer comprehensive QC testing, including: Restriction enzyme digestion analysis, RNA, host protein, and host DNA residue detection, Endotoxin testing, Sanger sequencing. Our goal is to deliver high-quality, high-standard plasmids while also providing customization and optimization to meet diverse client needs.

Service Highlights

• Scalable Preparation: From research-grade to industrial-grade plasmids, suitable for academic research, early-stage development, and industrial production.
• Flexible QC Options: Multiple default and optional testing items.
• Expert mRNA Plasmid Preparation: Ensures high-quality mRNA plasmid templates, validated for supercoiling, Poly(A) stability, and in vitro transcription (IVT).

Service Scope

• Industrial-grade and research-grade standard plasmid preparation
• Plasmid template preparation for mRNA production

Case Study

Process Step	Recovery (%)	Nano Conc. (ng/uL)	scDNA (%)	Endoxin (EU/mg pDNA)	HCP (ug/mg pDNA）	HCD (ug/mg pDNA）
Clarification and Filtration	/	/	/	＞10	0.034	/
AEX Elution	/	243.481	85	＞10	0.0032	2.4
HIC Elution	75	63.017	90.5	＜10	ND	0.02

Process Step	Recovery (%)	Nano Conc. (ng/uL)	scDNA (%)	Endoxin (EU/mg pDNA	HCP (ug/mg pDNA）	HCD (ug/mg pDNA）
Deep Filtration	/	/	59.3	/	0.034	/
Concentration&Buffer Exchange	/	1058.3	70.5	/	0.00075	2.85
AC Elution	79.2	173.726	84.6	/	0.0022	2.81
AEX Elution	86.7	368.488	93.4	/	ND	1.63

Service Process

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