Service introduction

By organically integrating the wisdom of predecessors and modern AI technology, the Hzymes R&D team has developed a series of software, which has achieved breakthrough and reproducible technical breakthroughs in expression system selection, expression increase, solubility modification, and stability modification. There are also forward-looking technical explorations and integration in the design of highly specific antigens, low-cost biological preparation of peptides, and enzyme library screening based on structure and algorithms. Our goal is to create a comprehensive protein intelligent body. The short-term goal is to achieve high-quality recombinant expression and modification of all proteins. The long-term vision is to design new tool enzymes according to the needs of catalytic function, that is, to deduce the catalytic center structure of enzyme proteins from the chemical mechanism of the catalyzed reaction, and then design highly stable auxiliary sequences to maintain the active center structure, so as to achieve enzyme protein design with high stability and high catalytic activity.

Service scope

Move towards our goal, protein intelligent body!

S1: Inclusion body becomes soluble:
Prokaryotic expression has the advantages of short cycle, low cost and easy operation. However, it also faces the situation of insoluble inclusion bodies when recombinantly expressing eukaryotic proteins. Hzymes not only has conventional technologies and methods to promote soluble expression, but also has its own unique solubility-promoting tags and soluble codon optimization programs to further improve our soluble expression success rate. At the same time, if the target protein is an enzyme and the customer needs the catalytic function of the protein, we can also improve the soluble expression ratio and absolute expression of the protein through sequence modification while maintaining the catalytic function of the protein.

S2: Low expression becomes high expression:
The low expression of recombinant protein is an obstacle on the road of scientific research. Based on the frequency of codon usage in different hosts and the relationship between bases in the sequence, the existing codon optimization algorithms are screened and organically combined one by one. Hzymes designed its own codon optimization program. In the E. coli system, according to current data, the high expression success rate of this program is as high as more than 98%. We ourselves can't believe that we are so lucky!

S3: High expression of short peptides in series:
Hzymes has developed an algorithm that combines the sequence of short peptides and the cleavage sites of various protein cleavage methods, comprehensively considers the cost and efficiency of each link such as expression, fermentation, and purification process development, and designs the recombinant expression of short peptides to achieve the ultimate reduction in the cost of short peptide production.

S4: Improved stability
Protein stability is one of the important characteristics of proteins. Naturally existing proteins sometimes cannot meet people's needs for them. Therefore, improving protein stability is a basic requirement of the enzyme preparation industry. Traditional enzyme methods not only have a long cycle, high cost, and low success rate. Based on structural analysis, directional model prediction and "high" throughput screening, Hzymes has greatly improved the efficiency of enzyme stability modification and has the ability to serve you efficiently.