Product Description
T7 RNA Polymerase (Thermostable) is suitable for in vitro transcription at higher temperature, using linear double-stranded DNA containing T7 promoter as template and NTPs as substrate to transcribe DNA sequences downstream of the promoter to obtain RNA. T7 RNA Polymerase (Thermostable) can also obtain biotin-, dye-, or radiolabeled RNAs using modified nucleosides as a substrate, and capped RNAs using cap structures or cap analogs as a substrate. The high-temperature reaction helps to reduce the formation of dsRNA byproducts and IVT product immunogenicity.
Product Information
| Product Name | Cat. No. | Size |
| T7 RNA Polymerase (Thermostable) 50 U/μL | HBP000350-1 | 5 KU/0.1mL |
| HBP000350-2 | 50 KU/1mL | |
| HBP000350-3 | 500 KU/10mL | |
| HBP000350-4 | 5000 KU/100mL |
Transportation and storage
Transportation under 0°C and storage at -25～ -15 °C.
Precaution
•The transcription reaction should be performed in an environment free of RNase contamination. Wearing gloves is advisable. The tips, tubes and water should be nuclease free.
•The transcription efficiency varies across different sequences, and gradient adjustments can be made to the reaction system.
