Product Overview

Bst DNA Polymerase V2 is an engineered DNA polymerase derived from Bacillus stearothermophilus DNA Polymerase I. The enzyme possesses strong strand-displacement activity and 5'→3' DNA polymerase activity, while lacking 5'→3' exonuclease activity. These properties make it highly suitable for isothermal amplification technologies such as Loop-Mediated Isothermal Amplification (LAMP) and other rapid nucleic acid detection workflows.

Hzymes Bst DNA Polymerase V2 delivers fast amplification speed, high tolerance to inhibitory salts, compatibility with dUTP/UDG contamination-control systems, and excellent long-term stability. WarmStart versions are also available for improved reaction specificity and reduced non-specific amplification during assay setup.

Key Features

Fast Amplification Performance

Delivers rapid strand-displacement amplification performance comparable to leading alternatives and maintains amplification efficiency after thermal acceleration testing.

High Salt Tolerance

Maintains robust amplification performance in the presence of potassium chloride and other common reaction inhibitors.

Excellent Long-Term Stability

Retains consistent enzymatic activity after extended storage under recommended conditions, with no significant activity loss observed over 12 months.

Compatible with dUTP/UDG Systems

Supports dUTP/UDG-based carryover contamination prevention workflows commonly used in molecular diagnostic assays.

High Purity

Manufactured with high purity standards and tested to be free from nuclease contamination and residual bacterial DNA, helping reduce the risk of false-positive results.

Applications

• Loop-Mediated Isothermal Amplification (LAMP)

• Isothermal nucleic acid amplification assays

• Molecular diagnostic reagent development

• Infectious disease detection

• Point-of-care testing (POCT)

• Food safety testing

• Environmental monitoring

• Rapid DNA detection workflows

• Research and industrial nucleic acid amplification applications

Performance Data

Fast Polymerization

Bst DNA Polymerase V2 performs almost the same speed compared against the competitor with the SARS-COV-2 N gene target. And better after 7 days thermal acceleration experiment.

Warmstart activity

Bst DNA Polymerase V2 could have higher blocking efficiency at 25°C, 37°C, and 40°C, and can release the activity even faster at 65°C than other competitors

Tolerance to Salt Inhibition

Bst DNA Polymerase V2 from Hzymes has better overall performance in the presence of potassium chloride.

Long-Term Stability

The activity test of Bst DNA Polymerase V2 (HMD7003) for 12 months under proper storage conditions. It has no activity change even after 12 months of storage.

FAQ

Does Bst V2 have reverse transcriptase activity?

No. Bst DNA Polymerase V2 has strong strand displacement DNA polymerase activity but does not have reverse transcriptase activity.

What are the key advantages of Bst V2?

A: It offers faster amplification, improved salt tolerance, and enhanced thermal stability compared with earlier Bst versions.

Can Bst V2 replace Taq polymerase?

No. Bst V2 is optimized for isothermal amplification (such as LAMP) and cannot replace Taq polymerase in PCR systems requiring high-temperature cycling.

What applications is Bst V2 suitable for?

It is widely used in LAMP, RCA, and other isothermal amplification technologies.

Does it have exonuclease activity?

No. It lacks 5’→3’ exonuclease activity.